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Glaucoma: HELP
Articles by Veluchamy A. Barathi
Based on 3 articles published since 2008

Between 2008 and 2019, V. A. Barathi wrote the following 3 articles about Glaucoma.
+ Citations + Abstracts
1 Review Assessment of flow dynamics in retinal and choroidal microcirculation. 2018

Wei, Xin / Balne, Praveen Kumar / Meissner, Kenith E / Barathi, Veluchamy A / Schmetterer, Leopold / Agrawal, Rupesh. ·National Healthcare Group Eye Institute, Tan Tock Seng Hospital, Singapore. · Singapore Eye Research Institute, Singapore. · Department of Physics, Swansea University, Swansea, UK. · Singapore Eye Research Institute, Singapore; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore; Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria; Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Austria. · National Healthcare Group Eye Institute, Tan Tock Seng Hospital, Singapore; Singapore Eye Research Institute, Singapore; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore. Electronic address: rupesh_agrawal@ttsh.com.sg. ·Surv Ophthalmol · Pubmed #29577954.

ABSTRACT: Alterations in ocular blood flow have been implicated in mechanisms that lead to vision loss in patients with various ocular disorders such as diabetic retinopathy, glaucoma, and age-related macular degeneration. Assessment of retinal and choroidal blood flow is also a window to evaluate systemic diseases that affect microvasculature. Quantification and qualification of the blood flow in the retina and choroid help us understand pathophysiology, stratify disease risk, and monitor disease progression in these disorders. Multiple methods are used by researchers for assessment of blood flow, but a gold standard is lacking. We review commonly used methods, both invasive and noninvasive, for evaluation of blood flow, including intravital microscopy, laser Doppler velocimetry, laser Doppler flowmetry, laser interferometry, confocal scanning laser Doppler flowmetry, laser speckle flowgraphy, Doppler optical coherence tomography, blue-field entoptic simulation, retinal vessel caliber assessment, optical coherence tomography angiography, retinal function imaging, color Doppler imaging, and scanning laser ophthalmoscope angiogram. As technology evolves, better evaluation of blood flow in various ocular and systemic diseases will likely bring new perspectives into clinical practice and translate to better diagnosis and treatment.

2 Article Sustained drug release in nanomedicine: a long-acting nanocarrier-based formulation for glaucoma. 2014

Natarajan, Jayaganesh V / Darwitan, Anastasia / Barathi, Veluchamy A / Ang, Marcus / Htoon, Hla Myint / Boey, Freddy / Tam, Kam C / Wong, Tina T / Venkatraman, Subbu S. ·School of Materials Science and Engineering, Nanyang Technological University , Blk N4.1, Nanyang Avenue, Singapore 639798. ·ACS Nano · Pubmed #24392729.

ABSTRACT: Therapeutic nanomedicine has concentrated mostly on anticancer therapy by making use of the nanosize for targeted therapy. Such nanocarriers are not expected to have sustained release of the bioactive molecule beyond a few days. There are other conditions where patients can benefit from sustained duration of action following a single instillation, but achieving this has been difficult in nanosized carriers. An important prerequisite for sustained delivery over several months is to have sufficiently high drug loading, without disruption or changes to the shape of the nanocarriers. Here we report on successful development of a drug-encapsulated nanocarrier for reducing intraocular pressure in a diseased nonhuman primate model and explain why it has been possible to achieve sustained action in vivo. The drug is a prostaglandin derivative, latanoprost, while the carrier is a nanosized unilamellar vesicle. The mechanistic details of this unique drug-nanocarrier combination were elucidated by isothermal titration calorimetry. We show, using Cryo-TEM and dynamic light scattering, that the spherical shape of the liposomes is conserved even at the highest loading of latanoprost and that specific molecular interactions between the drug and the lipid are the reasons behind improved stability and sustained release. The in vivo results clearly attest to sustained efficacy of lowering the intraocular pressure for 120 days, making this an excellent candidate to be the first truly sustained-release nanomedicine product. The mechanistic details we have uncovered should enable development of similar systems for other conditions where sustained release from nanocarriers is desired.

3 Article SPARC deficiency results in improved surgical survival in a novel mouse model of glaucoma filtration surgery. 2010

Seet, Li-Fong / Su, Roseline / Barathi, V A / Lee, Wing Sum / Poh, Rebekah / Heng, Yee Meng / Manser, Ed / Vithana, Eranga N / Aung, Tin / Weaver, Matt / Sage, E Helene / Wong, Tina T. ·Ocular Wound Healing and Therapeutics Group, Singapore Eye Research Institute, Singapore, Singapore. ·PLoS One · Pubmed #20195533.

ABSTRACT: Glaucoma is a disease frequently associated with elevated intraocular pressure that can be alleviated by filtration surgery. However, the post-operative subconjunctival scarring response which blocks filtration efficiency is a major hurdle to the achievement of long-term surgical success. Current application of anti-proliferatives to modulate the scarring response is not ideal as these often give rise to sight-threatening complications. SPARC (secreted protein, acidic and rich in cysteine) is a matricellular protein involved in extracellular matrix (ECM) production and organization. In this study, we investigated post-operative surgical wound survival in an experimental glaucoma filtration model in SPARC-null mice. Loss of SPARC resulted in a marked (87.5%) surgical wound survival rate compared to 0% in wild-type (WT) counterparts. The larger SPARC-null wounds implied that aqueous filtration through the subconjunctival space was more efficient in comparison to WT wounds. The pronounced increase in both surgical survival and filtration efficiency was associated with a less collagenous ECM, smaller collagen fibril diameter, and a loosely-organized subconjunctival matrix in the SPARC-null wounds. In contrast, WT wounds exhibited a densely packed collagenous ECM with no evidence of filtration capacity. Immunolocalization assays confirmed the accumulation of ECM proteins in the WT but not in the SPARC-null wounds. The observations in vivo were corroborated by complementary data performed on WT and SPARC-null conjunctival fibroblasts in vitro. These findings indicate that depletion of SPARC bestows an inherent change in post-operative ECM remodeling to favor wound maintenance. The evidence presented in this report is strongly supportive for the targeting of SPARC to increase the success of glaucoma filtration surgery.