Pick Topic
Review Topic
List Experts
Examine Expert
Save Expert
  Site Guide ··   
Hepatitis: HELP
Articles by Raghavendran Anantharam
Based on 6 articles published since 2010
(Why 6 articles?)
||||

Between 2010 and 2020, R. Anantharam wrote the following 6 articles about Hepatitis.
 
+ Citations + Abstracts
1 Article Evaluation of dried blood spots as a feasible alternative to plasma for the detection and quantification of hepatitis c virus in a tropical setting: A pilot study. 2019

Ranjan, Jai / Ponnuvel, Suresh / Fletcher, Gnanadurai John / Anantharam, Raghavendran / Radhakrishnan, Kalaivani / Jeyaseelan, Visalakshi / Abraham, Priya. ·Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India. · Department of Biostatistics, Christian Medical College, Vellore, Tamil Nadu, India. ·Indian J Med Microbiol · Pubmed #31424012.

ABSTRACT: Introduction: Confirmatory diagnosis of hepatitis C virus (HCV) infection (HCV RNA detection) is essential before start of the therapy. HCV RNA detection is not available in many parts of India. Shipment of plasma from distant places to referral laboratories may affect HCV RNA titres. Dried blood spots (DBS) provide an easy alternative for transporting samples to centres where HCV RNA testing is done. Aim: Evaluation of DBS as a feasible alternative to plasma for HCV diagnosis. Methods: In this cross-sectional study, 40 consecutive patients' blood samples were collected from patients referred from the Liver Clinic. Whole blood was spotted onto two Whatman 903 Results: The median log HCV RNA value (in log Conclusions: DBS can be used for sampling patients from distant resource-limited settings as an alternative to plasma for HCV RNA estimation. Larger studies are required to evaluate the feasibility of DBS in the Indian subcontinent, especially for HCV core antigen estimation.

2 Article Expanded diagnostic approach to hepatitis E virus detection in patients with acute-on-chronic liver failure: A pilot study. 2018

Steve, Runal John / Gnanadurai, Fletcher John / Anantharam, Raghavendran / Jeyaseelan, Visalakshi / Zachariah, Uday George / Goel, Ashish / Chundamannil, Eapen Eapen / Abraham, Priya. ·Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India. · Department of Biostatistics, Christian Medical College, Vellore, Tamil Nadu, India. · Department of Hepatology, Christian Medical College, Vellore, Tamil Nadu, India. ·Indian J Med Microbiol · Pubmed #30429393.

ABSTRACT: Introduction: Acute decompensation of pre-existing chronic liver disease (CLD), known as acute-on-chronic liver failure (ACLF), is associated with high mortality. Hepatitis E virus (HEV) as a potential cause was studied. Objectives: The objectives of this study are to evaluate the role of HEV in ACLF patients using an IgM anti-HEV antibody enzyme-linked immunosorbent assay (ELISA), HEV antigen ELISA, and a quantitative HEV polymerase chain reaction (PCR). Materials and Methods: In this prospective cross-sectional study, blood samples were collected from 50 ACLF (cases) as defined by the standard guidelines (APASL, 2014) and 50 patients with stable CLD (controls) from January 2015 to August 2016, after obtaining informed consent. Two IgM ELISAs (MP Diagnostics HEV IgM ELISA 3.0, Singapore and Wantai HEV IgM ELISA, Beijing, China) were compared using plasma from cases and controls. In addition, an HEV antigen detection by ELISA (Wantai, Beijing, China) and a real-time PCR for quantification of HEV RNA in plasma and stool were employed. Results: Ethanol was the leading cause of acute insult in ACLF (54%) cases. HEV infection accounted for 20% of cases. Ten ACLF patients (20%) had 1-3 markers of HEV versus two (4%) among controls (P = 0.0138). Among ACLF cases, one had HEV viraemia (403 IU/ml), faecal shedding (2790 IU/ml) and detectable HEV antigenaemia. Agreement between the two anti-HEV IgM ELISAs was 0.638 (kappa value). Conclusion: This study shows that alcohol is a major contributing factor for both underlying CLD and ACLF while HEV is the most common infectious cause for ACLF, suggesting a need for a vaccination in such patients, whenever made available.

3 Article Evaluation of reliability and performance of hepatitis B virus-e-antigen assays in tertiary care setting. 2018

Fletcher, Gnanadurai John / Anantharam, Raghavendran / Radhakrishnan, Kalaivani / Singh, Unnati / Karunakaran, Amaldev / Jeyaseelan, Visalakshi / Abraham, Priya. ·a Departments of Clinical Virology , Christian Medical College , Vellore , India. · b Departments of Clinical Virology, Biostatistics , Christian Medical College , Vellore , India. ·J Immunoassay Immunochem · Pubmed #30362912.

ABSTRACT: Hepatitis B virus-e-antigen (HBeAg) is a viral marker to assess hepatitis B virus (HBV) replication. We have evaluated the reliability of three commonly available HBeAg immunoassays using World Health Organization-International Standard and clinical samples. In addition the performance of enzyme immunoassays (EIAs) was assessed by kinetic binding and reagent exchange experiments. Analytical and diagnostic sensitivity were significantly different among HBeAg assays (P < 0.01). The affinity of capture/detector antibodies varied significantly between EIAs (P < 0.01). Our findings suggest that significant difference in the affinity of capture/detector antibodies to HBeAg may impact the overall performance and the reliability of currently available HBeAg assays in HBV diagnosis and management.

4 Article Cost-Effective In-House Neutralization Assay for the Confirmation of HBeAg. 2016

Fletcher, Gnanadurai John / Anantharam, Raghavendran / Radhakrishnan, Kalaivani / Karunakaran, Amaldev / Abraham, Priya. ·Department of Clinical Virology, Christian Medical College, Vellore, India. · Department of Clinical Virology, Christian Medical College, Vellore, India. priyaabraham@cmcvellore.ac.in. ·J Clin Lab Anal · Pubmed #27207380.

ABSTRACT: BACKGROUND & AIM: Hepatitis B virus-e-antigen (HBeAg) is an affordable viral marker to assess viral replication kinetics and response to antiviral therapy. In the absence of confirmatory assays, discrepant or false-positive HBeAg results are resolved by screening for other HBV markers. We standardized an in-house HBeAg neutralization assay (HBeAg-NT) to confirm HBeAg in clinical samples. METHODS: The performance and reliability of this assay were evaluated by first WHO International Standard for HBeAg (first WHO-IS HBeAg) from Paul Ehrlich Institute and clinical samples (n = 150) from chronic HBV carriers. Of these, 71 HBeAg-positive sera were used for HBeAg-NT. RESULTS: Concentrations spanning 0.25-10 U of first WHO-IS HBeAg and clinical samples (S/Co ranges from 1.00 to 10.00) were neutralized completely in the HBeAg-NT. CONCLUSIONS: HBeAg-NT is a simple, cost-effective, and reliable direct approach to confirm HBeAg in clinical samples which precludes the need for screening additional HBV markers in low resource settings.

5 Article Association of HLA and TNF polymorphisms with the outcome of HBV infection in the South Indian population. 2011

Fletcher, G J / Samuel, P / Christdas, J / Gnanamony, M / Ismail, A M / Anantharam, R / Eapen, C E / Chacko, M P / Daniel, D / Kannangai, R / Abraham, P. ·Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India. ·Genes Immun · Pubmed #21593777.

ABSTRACT: The role of host genetic factors in the pathogenesis and outcome of hepatitis B virus (HBV) infection is not well known. We assessed the association of HLA and TNF (rs361525, rs1800629, rs1799724, rs1800630 and rs1799964) polymorphisms with HBV outcome in the South Indian population. Association of HLA polymorphism was analyzed in 90 individuals from each group, that is, spontaneous recovery (SR) and chronic-HBV (C-HBV) infection. The role of TNF polymorphisms was evaluated in 150 subjects with SR and 137 patients with C-HBV infection. After adjusting for age and sex, HLA-DRB1*07:01 was strongly associated with chronicity (corrected P-value (pc) <0.005, odds ratio (OR) 3.76, 95% confidence interval (CI) 1.84-7.68). The rs1800630 genotype was associated with HBV outcome in codominant (pc<0.01, OR=1.99, 95% CI 1.30-3.05) and dominant (pc<0.01, OR=2.28, 95% CI 1.35-3.84) analyzing models after adjusting for age and sex. Similarly, the rs1799964 genotype was associated with HBV outcome in codominant (pc=0.01, OR=1.57, 95% CI 1.09-2.27) and dominant (pc<0.01, OR=2.21, 95% CI 1.27-3.83) analyzing models. Haplotype analysis (rs1799964/rs1800630/rs1799724/rs1800629/rs361525) revealed that the CACGG haplotype was strongly associated with C-HBV infection (P=0.0004). Our study suggests that inheritance of HLA and TNF polymorphisms might explain the outcome of HBV infection in the South Indian population.

6 Minor Low frequency of occult hepatitis B infection in anti-HBc seropositive blood donors: experience from a tertiary care centre in South India. 2012

Ismail, Ashrafali M / Devakumar, Seetha / Anantharam, Raghavendran / Fletcher, Gnanadurai J / Subramani, Thenmozhi / John, George T / Daniel, Dolly / Abraham, Priya. · ·Blood Transfus · Pubmed #22153686.

ABSTRACT: -- No abstract --