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Melanoma: HELP
Articles by Riccardo Bono
Based on 18 articles published since 2008
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Between 2008 and 2019, R. Bono wrote the following 18 articles about Melanoma.
 
+ Citations + Abstracts
1 Article Correlation between digital epiluminescence microscopy parameters and histopathological changes in lentigo maligna and solar lentigo: A dermoscopic index for the diagnosis of lentigo maligna. 2017

Annessi, Giorgio / Bono, Riccardo / Abeni, Damiano. ·Melanoma Unit, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico, Fondazione Luigi Maria Monti (IRCCS-FLMM), Rome, Italy; Laboratory of Dermatopathology, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico, Fondazione Luigi Maria Monti (IRCCS-FLMM), Rome, Italy. Electronic address: g.annessi@idi.it. · Melanoma Unit, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico, Fondazione Luigi Maria Monti (IRCCS-FLMM), Rome, Italy; Department of Dermatology, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico, Fondazione Luigi Maria Monti (IRCCS-FLMM), Rome, Italy. · Clinical Epidemiology Unit, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico, Fondazione Luigi Maria Monti (IRCCS-FLMM), Rome, Italy. ·J Am Acad Dermatol · Pubmed #28341252.

ABSTRACT: BACKGROUND: The clinical and dermoscopic differentiation between lentigo maligna (LM) and solar lentigo (SL)/initial seborrheic keratosis (SK) may be difficult. OBJECTIVE: Our aim was to identify digital epiluminescence microscopy (DELM)-specific criteria that can be helpful in distinguishing LM from SL/SK and to propose a new model of LM dermoscopic progression based on a study of DELM-histopathological correlation. METHODS: A total of 167 consecutive doubtful pigmented lesions of the head (105 LM and 62 SL/SK) were studied. DELM assessment was based on the presence or absence of 15 DELM parameters that were subsequently examined histologically. Statistical analysis was performed to determine which DELM parameters were most strongly associated with LM. RESULTS: The finding of at least 1 of 4 parameters (ie, brown globules, a "necklace" pigment network, an atypical pigment network, and dark-brown/blue-gray ribbonlike structures) showed to be an extremely sensitive (99%) and specific (83.9%) DELM criterion to discriminate between LM and SL/SK. LIMITATIONS: Our findings were obtained by examining medium-high magnification DELM images. CONCLUSIONS: The finding of 1 or more among the 4 above-mentioned DELM parameters allows for the correct identification of 99.0% of the LM lesions, and - when the score is 0 - the correct classification as non-LM, of 83.9% of the SL/SK lesions.

2 Article The effect of time to sentinel lymph node biopsy on cutaneous melanoma survival. 2016

Fortes, Cristina / Mastroeni, Simona / Caggiati, Alessio / Passarelli, Francesca / Zappalà, Alba / Capuano, Maria / Bono, Riccardo / Nudo, Maurizio / Marino, Claudia / Michelozzi, Paola. ·Epidemiology Unit, IDI-IRCCS-FLMM, Via Monti di Creta 104, 00167, Rome, Italy. Electronic address: c.fortes@idi.it. · Epidemiology Unit, IDI-IRCCS-FLMM, Via Monti di Creta 104, 00167, Rome, Italy. · Plastic Surgery Unit, IDI-IRCCS- FLMM, Rome, Italy. · Pathology Unit, IDI-IRCCS-FLMM, Rome, Italy. · Oncology Unit, IDI-IRCCS-FLMM, Rome, Italy. · Division Dermatology, Villa Paola; IDI-IRCCS-FLMM, Viterbo, Italy. · V Division Dermatology IDI-IRCCS-FLMM, Rome, Italy. · Department of Epidemiology of Lazio Regional Health Service, Rome, Italy. ·Am J Surg · Pubmed #27266393.

ABSTRACT: BACKGROUND: Whether timing of sentinel lymph node biopsy (SLNB) in cutaneous melanoma improves survival is not yet clear. The aim of this study was to investigate if the timing of SLNB influences long-term melanoma mortality. METHODS: A 10-year retrospective cohort study was conducted on 748 cutaneous melanoma patients who underwent excision of the SLN. Hazard ratios and 95% confidence intervals were estimated from Cox proportional hazards models. RESULTS: After adjusting for sex, age, Breslow thickness, mitotic rate, ulceration, and histologic type, patients who underwent early SLNB (≤30 days) and resulted positive on final pathology had a 3 times decreased risk of melanoma mortality (hazard ratio = .29; 95%confidence interval = .11 to .77) in comparison to patients who underwent delayed SLNB (≥31 days) and resulted positive on final pathology. CONCLUSIONS: Our findings suggest that early SLNB (≤30 days) improves melanoma survival.

3 Article Pigmented nodular melanoma: the predictive value of dermoscopic features using multivariate analysis. 2015

Pizzichetta, M A / Kittler, H / Stanganelli, I / Bono, R / Cavicchini, S / De Giorgi, V / Ghigliotti, G / Quaglino, P / Rubegni, P / Argenziano, G / Talamini, R / Anonymous1690828. ·Division of Medical Oncology - Preventive Oncology, Centro di Riferimento Oncologico, National Cancer Institute, Via Franco Gallini, 2, 33081, Aviano, Italy. · Department of Dermatology, University of Vienna, Vienna, Austria. · Skin Cancer Unit, Istituto Tumori Romagna (IRST), Meldola, Italy. · Istituto Dermopatico Immacolata, IRCCS, Rome, Italy. · Department of Dermatology, Fondazione Ospedale Maggiore Policlinico IRCCS, Milan, Italy. · Department of Dermatology, University of Florence, Florence, Italy. · Clinic of Dermatology, IRCCS San Martino - Ist, Genoa, Italy. · Dermatologic Clinic, Department of Medical Sciences, University of Turin, Turin, Italy. · Department of Dermatology, University of Siena, Siena, Italy. · Skin Cancer Unit, Arcispedale Santa Maria Nuova IRCCS, Reggio Emilia, Italy. · Unit of Epidemiology and Biostatistics, Centro di Riferimento Oncologico, National Cancer Institute, Via Franco Gallini, 2, 33081, Aviano, Italy. ·Br J Dermatol · Pubmed #25916655.

ABSTRACT: BACKGROUND: Nodular melanoma (NM), representing 10-30% of all melanomas, plays a major role in global mortality related to melanoma. Nonetheless, the literature on dermoscopy of NM is scanty. OBJECTIVES: To assess odds ratios (ORs) to quantify dermoscopic features of pigmented NM vs. pigmented superficial spreading melanoma (SSM), and pigmented nodular nonmelanocytic and benign melanocytic lesions. METHODS: To assess the presence or absence of global patterns and dermoscopic criteria, digitized images of 457 pigmented skin lesions from patients with a histopathological diagnosis of NM (n = 75), SSM (n = 93), and nodular nonmelanocytic and benign melanocytic lesions (n = 289; namely, 39 basal cell carcinomas, 85 seborrhoeic keratoses, 81 blue naevi, and 84 compound/dermal naevi) were retrospectively collected and blindly evaluated by three observers. RESULTS: Multivariate analysis showed that ulceration (OR 4.07), homogeneous disorganized pattern (OR 10.76), and homogeneous blue pigmented structureless areas (OR 2.37) were significantly independent prognostic factors for NM vs. SSM. Multivariate analysis of dermoscopic features of NM vs. nonmelanocytic and benign melanocytic lesions showed that the positive correlating features leading to a significantly increased risk of NM were asymmetric pigmentation (OR 6.70), blue-black pigmented areas (OR 7.15), homogeneous disorganized pattern (OR 9.62), a combination of polymorphous vessels and milky-red globules/areas (OR 23.65), and polymorphous vessels combined with homogeneous red areas (OR 33.88). CONCLUSIONS: Dermoscopy may be helpful in improving the recognition of pigmented NM by revealing asymmetric pigmentation, blue-black pigmented areas, homogeneous disorganized pattern and abnormal vascular structures, including polymorphous vessels, milky-red globules/areas and homogeneous red areas.

4 Article Amelanotic melanoma mimicking cutaneous epitheliomas. 2014

Napolitano, Monica / Didona, Biagio / Passarelli, Francesca / Annessi, Giorgio / Bono, Riccardo. ·Epiluminescence Microscopy Unit, Istituto Dermopatico dell'Immacolata-IRCCS, Rome, Italy. · I Dermatologic Unit, Istituto Dermopatico dell'Immacolata-IRCCS, Rome, Italy. · Histopathology Unit, Istituto Dermopatico dell'Immacolata-IRCCS, Rome, Italy. · Epiluminescence Microscopy Unit, Istituto Dermopatico dell'Immacolata-IRCCS, Rome, Italy. Electronic address: r.bono@idi.it. ·J Am Acad Dermatol · Pubmed #24629365.

ABSTRACT: -- No abstract --

5 Article One of the many faces of melanoma "incognito". 2014

Napolitano, Monica / Annessi, Giorgio / Nudo, Maurizio / Bono, Riccardo. ·Unit of Epiluminescence Microscopy, Istituto Dermopatico dell'Immacolata-IRCCS, Rome, Italy. · Unit of Histopathology, Istituto Dermopatico dell'Immacolata-IRCCS, Rome, Italy. · Unit of Epiluminescence Microscopy, Istituto Dermopatico dell'Immacolata-IRCCS, Rome, Italy. Electronic address: r.bono@idi.it. ·J Am Acad Dermatol · Pubmed #24528915.

ABSTRACT: -- No abstract --

6 Article Surgical management of suspicious melanocytic lesions in Italy. 2013

De Giorgi, Vincenzo / Ascierto, Paolo / Bono, Riccardo / Pimpinelli, Nicola / Chiarion-Sileni, Vanna / Palmieri, Giuseppe / Pizzichetta, Maria Antonietta / Testori, Alessandro / Stanganelli, Ignazio. ·Dermatologic Clinic, University of Florence, Florence, Italy. ·Dermatology · Pubmed #23736266.

ABSTRACT: OBJECTIVE: In melanoma, the surgical approach is important for both diagnosis and therapy. Although surgery is relatively simple, the methods should be performed by experts in melanoma management. We analyze the techniques and methods used in the Italian hospital network for suspicious skin lesions and confirmed melanomas. METHODS: A nationwide survey was conducted of a representative sample of 120 hospitals with ≥ 200 beds. RESULTS: Excision biopsies remove suspected melanomas. However, some approaches to excision margins and sentinel lymph node procedures differ from international protocols. Overall, 21% of centers perform excisional biopsy of a suspicious lesion using 1 cm margins, and 22% of centers perform sentinel node procedures concurrently with removal of primary melanoma. CONCLUSIONS: Standardized treatment protocols are needed for suspicious lesions and clinically evident melanoma, particularly regarding the critical aspect of excision margins. The sentinel lymph node procedure may be distorted by initial margins that are too wide.

7 Article Impact of mole mapping in the Italian health system. 2013

Stanganelli, Ignazio / Ascierto, Paolo / Bono, Riccardo / De Giorgi, Vincenzo / Pimpinelli, Nicola / Chiarion-Sileni, Vanna / Palmieri, Giuseppe / Pizzichetta, Maria Antonietta / Testori, Alessandro. ·Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori, IRCCS IRST, Meldola, Italy. ·Dermatology · Pubmed #23736265.

ABSTRACT: OBJECTIVE: To analyze routine clinical management of atypical melanocytic lesions through monitoring or surgery in Italian hospitals. METHODS: A nationwide survey of clinical practices was conducted. RESULTS: Digital monitoring is performed in most Italian hospitals and is preferred over excision for single atypical melanocytic lesions in 82% of hospitals. For multiple atypical lesions, 60% of high-volume hospitals prefer digital monitoring to surgical excision (40%). There is a statistically significant difference between high- and low-volume hospitals (60 vs. 39%; p = 0.003). Digital monitoring is performed at mean intervals of 4/5 months for both types of lesions. CONCLUSIONS: We show an asymmetric relation between application of the method and practical impact based on available clinical evidence. Although digital monitoring provides better characterization of the evolution of melanocytic lesions, our results indicate that the advantages and limitations of this method require further investigation.

8 Article Diagnostic services for melanoma in Italy. 2013

Stanganelli, Ignazio / Ascierto, Paolo / Bono, Riccardo / De Giorgi, Vincenzo / Pimpinelli, Nicola / Chiarion-Sileni, Vanna / Palmieri, Giuseppe / Pizzichetta, Maria Antonietta / Testori, Alessandro. ·Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori, IRCCS IRST, Meldola, Italy. ·Dermatology · Pubmed #23736263.

ABSTRACT: OBJECTIVE: To evaluate organizational structure and diagnostic procedures used by the Italian hospital network for identifying cutaneous melanoma. METHODS: A nationwide survey of a representative sample of centers was conducted. RESULTS: Diagnosis occurs mainly in ambulatory dermatology clinics (91%). In all high-volume hospitals, clinical and dermoscopic examination is available at first consultation or as an additional service, compared to 89% of low-volume hospitals. Computer-assisted videodermoscopy is available in 75% of hospitals, with a statistically significant difference between high- and low-volume hospitals (86 vs. 62%; p < 0.001). First consultation is generally an integrated clinical/dermoscopic evaluation (55% of high-volume centers vs. 47% of low-volume hospitals); digital evaluation is available for monitoring suspicious lesions and high-risk patients in 25% of high-volume centers versus 19% of low-volume centers. CONCLUSIONS: The organizational structure and diagnostic procedures in Italian hospitals are in line with modern diagnostic procedures for early diagnosis of melanoma. Dermatologists have a central role in managing diagnosis of primitive melanoma.

9 Article Dermoscopic evaluation of nodular melanoma. 2013

Menzies, Scott W / Moloney, Fergal J / Byth, Karen / Avramidis, Michelle / Argenziano, Giuseppe / Zalaudek, Iris / Braun, Ralph P / Malvehy, Josep / Puig, Susana / Rabinovitz, Harold S / Oliviero, Margaret / Cabo, Horacio / Bono, Riccardo / Pizzichetta, Maria A / Claeson, Magdalena / Gaffney, Daniel C / Soyer, H Peter / Stanganelli, Ignazio / Scolyer, Richard A / Guitera, Pascale / Kelly, John / McCurdy, Olivia / Llambrich, Alex / Marghoob, Ashfaq A / Zaballos, Pedro / Kirchesch, Herbert M / Piccolo, Domenico / Bowling, Jonathan / Thomas, Luc / Terstappen, Karin / Tanaka, Masaru / Pellacani, Giovanni / Pagnanelli, Gianluca / Ghigliotti, Giovanni / Ortega, Blanca Carlos / Crafter, Greg / Ortiz, Ana María Perusquía / Tromme, Isabelle / Karaarslan, Isil Kilinc / Ozdemir, Fezal / Tam, Anthony / Landi, Christian / Norton, Peter / Kaçar, Nida / Rudnicka, Lidia / Slowinska, Monika / Simionescu, Olga / Di Stefani, Alessandro / Coates, Elliot / Kreusch, Juergen. ·Sydney Melanoma Diagnostic Centre, Sydney Cancer Centre, Royal Prince Alfred Hospital, Camperdown, NSW 2050, Australia. scott.menzies@sswahs.nsw.gov.au ·JAMA Dermatol · Pubmed #23553375.

ABSTRACT: IMPORTANCE: Nodular melanoma (NM) is a rapidly progressing potentially lethal skin tumor for which early diagnosis is critical. OBJECTIVE: To determine the dermoscopy features of NM. DESIGN: Eighty-three cases of NM, 134 of invasive non-NM, 115 of nodular benign melanocytic tumors, and 135 of nodular nonmelanocytic tumors were scored for dermoscopy features using modified and previously described methods. Lesions were separated into amelanotic/hypomelanotic or pigmented to assess outcomes. SETTING: Predominantly hospital-based clinics from 5 continents. MAIN OUTCOME MEASURES: Sensitivity, specificity, and odds ratios for features/models for the diagnosis of melanoma. RESULTS: Nodular melanoma occurred more frequently as amelanotic/hypomelanotic (37.3%) than did invasive non-NM (7.5%). Pigmented NM had a more frequent (compared with invasive non-NM; in descending order of odds ratio) symmetrical pigmentation pattern (5.8% vs 0.8%), large-diameter vessels, areas of homogeneous blue pigmentation, symmetrical shape, predominant peripheral vessels, blue-white veil, pink color, black color, and milky red/pink areas. Pigmented NM less frequently displayed an atypical broadened network, pigment network or pseudonetwork, multiple blue-gray dots, scarlike depigmentation, irregularly distributed and sized brown dots and globules, tan color, irregularly shaped depigmentation, and irregularly distributed and sized dots and globules of any color. The most important positive correlating features of pigmented NM vs nodular nonmelanoma were peripheral black dots/globules, multiple brown dots, irregular black dots/globules, blue-white veil, homogeneous blue pigmentation, 5 to 6 colors, and black color. A model to classify a lesion as melanocytic gave a high sensitivity (>98.0%) for both nodular pigmented and nonnodular pigmented melanoma but a lower sensitivity for amelanotic/hypomelanotic NM (84%). A method for diagnosing amelanotic/hypomelanotic malignant lesions (including basal cell carcinoma) gave a 93% sensitivity and 70% specificity for NM. CONCLUSIONS AND RELEVANCE: When a progressively growing, symmetrically patterned melanocytic nodule is identified, NM needs to be excluded.

10 Article Negative pigment network: an additional dermoscopic feature for the diagnosis of melanoma. 2013

Pizzichetta, Maria A / Talamini, Renato / Marghoob, Ash A / Soyer, H Peter / Argenziano, Giuseppe / Bono, Riccardo / Corradin, M Teresa / De Giorgi, Vincenzo / Gonzalez, Marian A / Kolm, Isabel / Kopf, Andrew W / Malvehy, Joseph / Nami, Niccolò / Oliviero, Margaret / Pellacani, Giovanni / Puig, Susana / Rabinovitz, Harold / Rubegni, Pietro / Seidenari, Stefania / Stanganelli, Ignazio / Veronesi, Andrea / Zalaudek, Iris / Zampieri, Pierfrancesco / Menzies, Scott W. ·Centro di Riferimento Oncologico, National Cancer Institute, Aviano, Italy. Electronic address: pizzichetta@cro.it. · Centro di Riferimento Oncologico, National Cancer Institute, Aviano, Italy. · Dermatology Section, Memorial Sloan Kettering Cancer Center, New York, New York. · Dermatology Research Center, University of Queensland, School of Medicine, Princess Alexandra Hospital, Brisbane, Australia. · Dermatology and Skin Cancer Unit, Arcispedale Santa Maria Nuova, Istituto di Ricovero e Cura a Carattere Scientifico(IRCCS), Reggio Emilia, Italy. · Istituto Dermopatico Immacolata, IRCCS, Rome, Italy. · Division of Dermatology, Pordenone Hospital, Pordenone, Italy. · Department of Dermatology, University of Florence, Florence, Italy. · Division of Dermatology, Merano Hospital, Merano, Italy. · Department of Dermatology, University of Miami, Miami, Florida. · New York University School of Medicine, New York, New York. · Melanoma Unit, Department of Dermatology, Hospital Clinic, Institut d'Investigacions Biomediques August Pi i Sunyer, Barcelona, Spain. · Department of Dermatology, University of Siena, Siena, Italy. · Department of Dermatology, University of Modena and Reggio Emilia, Modena and Reggio Emilia, Italy. · Skin Cancer Unit, Istituto Tumori Romagna, Meldola, Italy. · Medical University of Graz, Graz, Austria. · Sydney Melanoma Diagnostic Center, Royal Prince Alfred Hospital and Discipline of Dermatology, University of Sydney, Sydney, Australia. ·J Am Acad Dermatol · Pubmed #23062610.

ABSTRACT: BACKGROUND: The negative pigment network (NPN) is seen as a negative of the pigmented network and it is purported to be a melanoma-specific structure. OBJECTIVES: We sought to assess the frequency, sensitivity, specificity, and odds ratios (ORs) of NPN between melanoma cases and a group of control lesions. METHODS: Digitalized images of skin lesions from 679 patients with histopathological diagnosis of dermatofibroma (115), melanocytic nevus (220), Spitz nevus (139), and melanoma (205) were retrospectively collected and blindly evaluated to assess the presence/absence of NPN. RESULTS: The frequency of occurrence of NPN was higher in the melanoma group (34.6%) than in Spitz nevus (28.8%), melanocytic nevus (18.2%), and dermatofibroma (11.3%) groups. An OR of 1.8 emerged for the diagnosis of melanoma in the presence of NPN as compared with nonmelanoma diagnosis. Conversely, for melanocytic nevi and dermatofibromas the OR was very low (0.5 and 0.3, respectively). For Spitz nevi the OR of 1.1 was not statistically significant. When comparing melanoma with dermatofibroma, melanocytic nevus, and Spitz nevus, we observed a significantly higher frequency of multicomponent pattern (68.1%), asymmetric pigmentation (92.9%), irregularly distributed NPN (87.3%), and peripheral location of NPN (66.2%) in melanomas. LIMITATIONS: Further studies can provide the precise dermoscopic-histopathologic correlation of NPN in melanoma and other lesions. CONCLUSIONS: The overall morphologic pattern of NPN, such as the irregular distribution and the peripheral location of NPN, along with the multicomponent pattern and the asymmetric pigmentation could be used as additional features in distinguishing melanoma from Spitz nevus and other benign lesions.

11 Article Dermoscopy and digital dermoscopy analysis of palmoplantar 'equivocal' pigmented skin lesions in Caucasians. 2012

Rubegni, P / Cevenini, G / Nami, N / Argenziano, G / Saida, T / Burroni, M / Bono, R / Quaglino, P / Barbini, P / Miracco, C / Lamberti, A / Fimiani, M. ·Dermatology Section, Department of Clinical Medicine and Immunological Science, University of Siena, Siena, Italy. rubegni@unisi.it ·Dermatology · Pubmed #23182753.

ABSTRACT: BACKGROUND/AIM: The diagnosis of palmoplantar melanoma is often delayed and misdiagnosis is common, due to frequently unusual clinical presentation. We used a digital dermoscopy analyzer with a series of palmoplantar pigmented skin lesions (PP-PSL), and we compared sensitivity, specificity and diagnostic accuracy obtained with digital dermoscopy analysis (DDA) and classical dermoscopy. METHODS: Digital dermoscopy images of 107 PP-PSL were retrospectively obtained from the database of images of 3 Italian centers. The lesions (25 melanomas and 82 nevi) were all removed because of the presence of clinical and/or dermoscopic suspicious features. All digital images were analyzed using appropriate algorithms, and the diagnostic accuracy of the model was calculated. For comparison, dermoscopic images were clinically evaluated by two dermatologists and the Cohen ĸ concordance with DDA was calculated. RESULTS: The stepwise logistic regression analysis selected only 5 parameters out of 49. The logistic model achieved a sensitivity of 96% and a specificity of 87.8%. The Cohen ĸ concordance, evaluated by the Landis and Koch scale, supplied a substantial agreement between dermoscopy and DDA. CONCLUSIONS: DDA might be a useful diagnostic instrument in the evaluation of preselected PP-PSL. However, these findings should be confirmed in a formal clinical trial.

12 Article Objective melanoma progression. 2011

Rubegni, Pietro / Burroni, Marco / Nami, Niccolò / Cevenini, Gabriele / Bono, Riccardo / Sbano, Paolo / Fimiani, Michele. ·Department of Clinical Medicine and Immunological Science, Dermatology Section, University of Siena, Siena, Italy. rubegni@unisi.it ·Skin Res Technol · Pubmed #20923468.

ABSTRACT: BACKGROUND/PURPOSE: Many aspects of the natural history of malignant melanoma (MM) are still unclear, specifically its appearance at onset and particularly how it changes in time. The purpose of our study was to retrospectively determine objective changes in melanoma over a 3-24-month observation period. MATERIALS AND METHODS: Our study was carried out in two Italian dermatology centers. Digital dermoscopy analyzers (DB-Mips System) were used to retrospectively evaluate dermoscopic images of 59 MM (with no initial clinical aspects suggesting melanoma) under observation for 3-24 months. The analyzer evaluates 49 parameters grouped into four categories: geometries, colors, textures and islands of color. Multivariate analysis of variance for repeated measures was used to evaluate the statistical significance of the changes in the digital dermoscopy variables of melanomas. RESULTS: Within-lesion analysis indicated that melanomas increased in dimension (Area, Minimum, and Maximum Diameter), manifested greater disorganization of the internal components (Red, Green and Blue Multicomponent, Contrast, and Entropy) and increased in clusters of milky pink color (Light Red Area). CONCLUSION: Analysis of the parameters of our model and statistical analysis enabled us to interpret/identify the most significant factors of melanoma modification, providing quantitative insights into the natural history of this cutaneous malignancy.

13 Article Objective follow-up of atypical melanocytic skin lesions: a retrospective study. 2010

Rubegni, Pietro / Cevenini, Gabriele / Burroni, Marco / Bono, Riccardo / Sbano, Paolo / Biagioli, Maurizio / Risulo, Massimiliano / Nami, Niccolò / Perotti, Roberto / Miracco, Clelia / Fimiani, Michele. ·Department of Clinical Medicine and Immunological Science, Policlinico "Le Scotte", Siena, Italy. rubegni@unisi.it ·Arch Dermatol Res · Pubmed #20411393.

ABSTRACT: Various authors have suggested that information from longitudinal observation (follow-up) of dynamic changes in atypical melanocytic pigmented skin lesions (MPSL) could enable identification of early malignant melanoma escaping initial observation due to an absence of specific clinical and dermoscopic features. The aim of our retrospective study was to determine the existence of numerical variables regarding changes in MPSL that could be useful to differentiate early melanomas and atypical nevi. The study was carried out in two Italian dermatology Centres. Digital dermoscopy analyzers (DB-Mips System) were used to evaluate dermoscopic images of 94 equivocal pigmented skin lesions under observation for 6-12 months and then excised because of changes across time (29 melanomas and 65 nevi). The analyzer evaluates 49 parameters grouped into four categories: geometries, colours, textures and islands of colour. The ROC curve designed on the 49 digital dermoscopy analysis parameters showed good accuracy. At sensitivity (SE) = specificity (SP), it correctly classified 89.3% of cases. When objective pigmented skin lesion parameters were considered together with their objective changes over 6-12 months, a decisive increase in discrimination capacity was obtained. At SE = SP accuracy was 96.3%. Analysis of the parameters of our model and statistical analysis enabled us to interpret/identify the most significant factors of modification and differentiation of lesions, providing quantitative insights into the diagnosis of equivocal MPSL and demonstrating the utility of objective/numerical follow-up.

14 Article Role of the EGF +61A>G polymorphism in melanoma pathogenesis: an experience on a large series of Italian cases and controls. 2009

Casula, Milena / Alaibac, Mauro / Pizzichetta, Maria A / Bono, Riccardo / Ascierto, Paolo A / Stanganelli, Ignazio / Canzanella, Sergio / Palomba, Grazia / Zattra, Edoardo / Anonymous3140634 / Palmieri, Giuseppe. ·Unit of Cancer Genetics, Institute of Biomolecular Chemistry, Consiglio Nazionale delle Ricerche, Sassari, Italy. casulam@yahoo.it ·BMC Dermatol · Pubmed #19624835.

ABSTRACT: BACKGROUND: A single nucleotide polymorphism (61A>G) in the epidermal growth factor (EGF) gene has been implicated in both melanoma pathogenesis and increased melanoma risk. To further evaluate this association, we conducted a case-control study in a clinic-based Italian population. METHODS: Individuals with less than 10 (N = 127) or more than 100 (N = 128) benign nevi, and patients with cutaneous melanoma (N = 418) were investigated for the EGF +61A>G polymorphism, using an automated sequencing approach. RESULTS: Overall, no difference in EGF genotype frequencies was observed among subjects with different number of nevi as well as when non-melanoma healthy controls were compared with the melanoma patients. However, a heterogeneous distribution of the frequencies of the G/G genotype was detected among cases and controls originating from North Italy (21.1 and 18.3%, respectively) vs. those from South Italy (12.6 and 17.1%, respectively). CONCLUSION: Our findings further suggest that EGF +61A>G polymorphism may have a limited impact on predisposition and/or pathogenesis of melanoma and its prevalence may vary in different populations.

15 Article CDKN2A and MC1R analysis in amelanotic and pigmented melanoma. 2009

Ghiorzo, Paola / Pastorino, Lorenza / Pizzichetta, Maria A / Bono, Riccardo / Queirolo, Paola / Talamini, Renato / Annessi, Giorgio / Bruno, William / Nasti, Sabina / Gargiulo, Sara / Battistuzzi, Linda / Sini, Maria C / Palmieri, Giuseppe / Scarrà, Giovanna Bianchi / Anonymous40720625. ·Dipartimento di Oncologia, Biologia e Genetica, Università di Genova, Genova, Italy. paola.ghiorzo@unige.it ·Melanoma Res · Pubmed #19339902.

ABSTRACT: Amelanotic melanoma (AM) is a rare subtype of melanoma with little or no clinically visible pigment; it is more difficult to diagnose than pigmented melanoma (PM), and has a worse prognosis. In the attempt to find a genetic explanation for the distinction between AM and PM, we conducted a case-case study, matching AM and PM patients, and testing them for germline mutations in high- (p16INK4A, p14ARF, CDK4) and low-penetrance (MC1R) melanoma susceptibility genes. Similar CDKN2A mutations were found in both sets of melanomas. A p14ARF splice germline mutation was detected for the first time in an Italian family with AM. This rare mutation, which has been described only once previously, may be involved in predisposition to the amelanotic phenotype in combination with germline MC1R variants and coordinate somatic expression of pigmentation genes and their regulators.

16 Article Dermoscopic evaluation of amelanotic and hypomelanotic melanoma. 2008

Menzies, Scott W / Kreusch, Juergen / Byth, Karen / Pizzichetta, Maria A / Marghoob, Ashfaq / Braun, Ralph / Malvehy, Josep / Puig, Susana / Argenziano, Giuseppe / Zalaudek, Iris / Rabinovitz, Harold S / Oliviero, Margaret / Cabo, Horacio / Ahlgrimm-Siess, Verena / Avramidis, Michelle / Guitera, Pascale / Soyer, H Peter / Ghigliotti, Giovanni / Tanaka, Masaru / Perusquia, Ana M / Pagnanelli, Gianluca / Bono, Riccardo / Thomas, Luc / Pellacani, Giovanni / Langford, David / Piccolo, Domenico / Terstappen, Karin / Stanganelli, Ignazio / Llambrich, Alex / Johr, Robert. ·Faculty of Medicine, University of Sydney, Sydney Melanoma Diagnostic Centre, Royal Prince Alfred Hospital, Camperdown, NSW 2050, Australia. scott.menzies@email.cs.nsw.gov.au ·Arch Dermatol · Pubmed #18794455.

ABSTRACT: OBJECTIVE: To determine the predictive dermoscopic features of amelanotic and hypomelanotic melanoma. DESIGN: A total of 105 melanomas (median Breslow thickness, 0.76 mm), 170 benign melanocytic lesions, and 222 nonmelanocytic lesions lacking significant pigment (amelanotic, partially pigmented, and light colored) were imaged using glass-plate dermoscopy devices and scored for 99 dermoscopic features. Diagnostic models were derived from and tested on independent randomly selected lesions. SETTING: Predominantly hospital-based clinics from 5 continents. MAIN OUTCOME MEASURES: Sensitivity, specificity, and odds ratios for individual features and models for the diagnosis of melanoma and malignancy. RESULTS: The most significant negative predictors of melanoma were having multiple (>3) milialike cysts (odds ratio, 0.09; 95% confidence interval, 0.01-0.64), comma vessels with a regular distribution (0.10; 0.01-0.70), comma vessels as the predominant vessel type (0.16; 0.05-0.52), symmetrical pigmentation pattern (0.18; 0.09-0.39), irregular blue-gray globules (0.20; 0.05-0.87), and multiple blue-gray globules (0.28; 0.10-0.81). The most significant positive predictors were having a blue-white veil (odds ratio,13; 95% confidence interval, 3.9-40.0), scarlike depigmentation (4.4; 2.4-8.0), multiple blue-gray dots (3.5; 1.9-6.4), irregularly shaped depigmentation (3.3; 2.0-5.3), irregular brown dots/globules (3.2; 1.8-5.6), 5 to 6 colors (3.2; 1.6-6.3), and predominant central vessels (3.1; 1.6-6.0). A simple model distinguishing melanomas from all nonmelanomas had a sensitivity of 70% and a specificity of 56% in the test set. A model distinguishing all malignant lesions from benign lesions had a sensitivity of 96% and a specificity of 37%. Conclusion Although the diagnostic accuracy of dermoscopy for melanoma lacking significant pigment is inferior to that of more pigmented lesions, features distinguishing the former from benign lesions can be visualized on dermoscopic evaluation.

17 Article Identification of melanoma with a gas sensor array. 2008

D'Amico, A / Bono, R / Pennazza, G / Santonico, M / Mantini, G / Bernabei, M / Zarlenga, M / Roscioni, C / Martinelli, E / Paolesse, R / Di Natale, C. ·Department of Electronic Engineering, University of Rome Tor Vergata, Roma, Italy. ·Skin Res Technol · Pubmed #18412567.

ABSTRACT: BACKGROUND/PURPOSE: The relationship between diseases and alterations of the airborne chemicals emitted from the body has been found in many different pathologies and in particular for various forms of cancer. Metabolism of cancer cells is greatly altered during their lifetime; then, modification of chemicals is supposed to be large around cancer tissues. Positive hints in this direction were provided, as an example, on studying the breath composition of lung cancer-affected subjects. Besides the conventional analytical approaches, in recent years sensor arrays were also applied to these researches considering the chemical composition changes as those occurring in other applications such as for instance, those dealing with food quality measurements. METHODS: In this paper, the first application of sensor arrays to study the differentiation between melanomas and nevi, namely malignant and benign affection of melanocytary cells, respectively, is presented and discussed. The localization of lesions on the skin surface made possible the utilization of differential measurements aimed at capturing the differences between two adjacent skin regions. This approach strongly reduces the influence of skin headspace variability due to the peculiar subjective odour background and the skin odour variability. The measurement campaign involved 40 cases; 10 of these were diagnosed melanomas referred to surgical intervention. Nine of these diagnoses were further confirmed by histological examinations of the removed tissue and one was a false positive. RESULTS: The differences in the chemical composition of headspace were verified with a gas-chromatographic investigation, and the classification of electronic nose data provided an estimated cross-validated accuracy of the same order of magnitude as the currently used diagnostic instruments. CONCLUSION: Electronic nose sensors have been shown to have good sensitivity towards volatile organic compounds emitted by skin lesions, and the method seems to be effective for malign lesions identification. The results presented in this paper encourage a second experimental campaign with a larger number of participants and a systematic use of gas chromatography mass spectrometer technology in order to identify some possible melanoma biomarkers.

18 Minor Dermoscopic diagnosis of amelanotic/hypomelanotic melanoma. 2017

Pizzichetta, M A / Kittler, H / Stanganelli, I / Ghigliotti, G / Corradin, M T / Rubegni, P / Cavicchini, S / De Giorgi, V / Bono, R / Alaibac, M / Astorino, S / Ayala, F / Quaglino, P / Pellacani, G / Argenziano, G / Guardoli, D / Specchio, F / Serraino, D / Talamini, R / Anonymous24890882. ·Division of Medical Oncology - Preventive Oncology, Centro di Riferimento Oncologico, National Cancer Institute, Aviano, Italy. · Department of Dermatology, Medical University of Vienna, Vienna, Austria. · Skin Cancer Unit, Istituto Tumori Romagna (IRST), Meldola, Department of Dermatology, University of Parma, Italy. · IRCCS San Martino - 1st Clinic of Dermatology, Genova, Italy. · Division of Dermatology, Pordenone Hospital, Pordenone, Italy. · Department of Dermatology, University of Siena, Siena, Italy. · Department of Dermatology, Fondazione Ospedale Maggiore Policlinico IRCCS, Milan, Italy. · Department of Dermatology, University of Florence, Florence, Italy. · Istituto Dermopatico Immacolata, IRCCS, Rome, Italy. · Department of Dermatology, University of Padova, Italy. · Division of Dermatology, Celio Hospital, Rome, Italy. · National Cancer Institute, 'Fondazione G. Pascale'-IRCCS, Naples, Italy. · Dermatologic Clinic, Department of Medical Sciences, University of Torino, Torino, Italy. · Department of Dermatology, University of Modena and Reggio Emilia, Modena, Italy. · Dermatology Unit, Second University of Naples, Naples, Italy. · Skin Cancer Unit, Arcispedale Santa Maria Nuova IRCCS, Reggio Emilia, Italy. · Unit of Epidemiology and Biostatistics, Centro di Riferimento Oncologico, National Cancer Institute, Aviano, Italy. ·Br J Dermatol · Pubmed #27681347.

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