Pick Topic
Review Topic
List Experts
Examine Expert
Save Expert
  Site Guide ··   
Melanoma: HELP
Articles by Mohib W. Morcos
Based on 5 articles published since 2010
(Why 5 articles?)
||||

Between 2010 and 2020, Mohib Morcos wrote the following 5 articles about Melanoma.
 
+ Citations + Abstracts
1 Article Effects of Long-term Serial Passaging on the Characteristics and Properties of Cell Lines Derived From Uveal Melanoma Primary Tumors. 2016

Mouriaux, Frédéric / Zaniolo, Karine / Bergeron, Marjorie-Allison / Weidmann, Cindy / De La Fouchardière, Arnaud / Fournier, Frédéric / Droit, Arnaud / Morcos, Mohib W / Landreville, Solange / Guérin, Sylvain L. ·Centre universitaire d'ophtalmologie-Recherche (CUO-Recherche), Axe médecine régénératrice, Hôpital du Saint-Sacrement, Centre de Recherche FRQS du CHU de Québec-Université Laval, Québec, Canada 2Département d'ophtalmologie, Faculté de Médecine, Université Laval, Québec, Canada 3CNRS, UMR 6301 ISTCT, CERVOxy. GIP CYCERON, Caen, France 4CHU de Rennes, Service d'Ophtalmologie, France. · Centre universitaire d'ophtalmologie-Recherche (CUO-Recherche), Axe médecine régénératrice, Hôpital du Saint-Sacrement, Centre de Recherche FRQS du CHU de Québec-Université Laval, Québec, Canada 5Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX, Québec, Canada. · Département de biopathologie, Centre Léon Bérard, Lyon, France. · Département de médecine moléculaire, CHUL, Centre de Recherche FRQS du CHU de Québec-Université Laval, Québec, Canada. · Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX, Québec, Canada 8Service de pathologie, Hôpital du Saint-Sacrement, Centre de Recherche FRQS du CHU de Québec-Université Laval, Québec, Canada. · Centre universitaire d'ophtalmologie-Recherche (CUO-Recherche), Axe médecine régénératrice, Hôpital du Saint-Sacrement, Centre de Recherche FRQS du CHU de Québec-Université Laval, Québec, Canada 2Département d'ophtalmologie, Faculté de Médecine, Université Laval, Québec, Canada 5Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX, Québec, Canada. ·Invest Ophthalmol Vis Sci · Pubmed #27723895.

ABSTRACT: Purpose: Development of liver metastasis remains the most common cause of mortality in uveal melanoma (UM). A few cell lines cultured from primary UM tumors have been used widely to investigate the pathobiology of UM. However, the translation of basic knowledge to the clinic for the treatment of the metastatic disease has remained incremental at best. In this study, we examined whether the properties of UM cell lines at various passages were similar to their corresponding primary tumors. Methods: Gene expression profiling by microarray was performed on UM primary tumors and derived cell lines cultured at varying passages. Expression of UM protein markers was monitored by immunohistochemical analyses and Western blotting. The in vivo tumorigenic properties of UM cultures were evaluated using athymic nude mice. Results: Cell passaging severely reduced the expression of genes encoding markers typical of UM, including those of the prognostic gene signature. Marked differences between gene expression profiles of primary tumors and cell lines could be linked to the infiltrating immune and stromal cells in situ. In addition, the tumorigenic properties of UM cell lines also increased with cell passaging in culture as evaluated by their subcutaneous injection into athymic mice. Conclusions: Together, these findings demonstrate that the short-term UM primary cultures exhibit molecular features that resemble the respective surgical material and, thus, represent the best model for in vitro-assessed cancer treatments.

2 Article Balloon cell nevus of the iris. 2014

Morcos, Mohib W / Odashiro, Alexandre / Bazin, Richard / Pereira, Patricia Rusa / O'Meara, Aisling / Burnier, Miguel N. ·Department of Pathology, Laval University, Canada. · Department of Pathology and Molecular Medicine, McMaster University, Canada. Electronic address: alexandreodashiro@gmail.com. · Department of Ophthalmology, Laval University, Canada. · Henry C. Witelson Ocular Pathology Laboratory, McGill University, Canada. · Department of Pathology and Molecular Medicine, McMaster University, Canada. ·Pathol Res Pract · Pubmed #24951244.

ABSTRACT: CASE REPORT: A 55 year-old man presented a pigmented elevated lesion in the right iris since the age of 12 years old. The lesion had been growing for the past 2 years and excision was performed. Histopathological examination showed a balloon cell nevus composed of clear and vacuolated cells without atypia. A typical spindle cell nevus of the iris was also observed. The differential diagnosis included xanthomatous lesions, brown adipocyte or other adipocytic lesions, clear cell hidradenoma, metastatic clear cell carcinoma of the kidney and clear cell sarcoma. The tumor was positive for Melan A, S100 protein and HMB45. CONCLUSION: Balloon cell nevus of the iris is rare but should be considered in the differential diagnosis of melanocytic lesions of the iris.

3 Article Increased HIF-1α expression correlates with cell proliferation and vascular markers CD31 and VEGF-A in uveal melanoma. 2014

Mouriaux, Frédéric / Sanschagrin, François / Diorio, Caroline / Landreville, Solange / Comoz, François / Petit, Edwige / Bernaudin, Myriam / Rousseau, Alain P / Bergeron, Dan / Morcos, Mohib. ·Centre National de Recherche Scientifique, Unité Mixte de Recherche 6301, Imagerie et Stratégies Thérapeutiques des pathologies Cérébrales et Tumorales, CERXOxy, GIP Cycéron, Caen, France. ·Invest Ophthalmol Vis Sci · Pubmed #24481264.

ABSTRACT: PURPOSE: Overexpression of hypoxia inducible factor-1 α (HIF-1α) has been found in several cancers and is thought to correlate with aggressive disease. The purpose of our study was to investigate the influence of HIF-1α on clinical outcome in uveal melanoma (UM) along with proliferative (MIB-1) and vascular (CD31, VEGF-A) markers. METHODS: A retrospective analysis was carried out on UM tumors from 88 patients. HIF-1α, MIB-1, CD31, and VEGF-A expression, as well as necrosis, were assessed by immunohistochemistry and hematoxylin/eosin on paraffin-embedded UM tumor sections by using a tissue microarray. The bivariate analysis involving HIF-1α expression and clinicopathologic covariates was performed by using the χ(2) test. The association of clinicopathologic covariates and HIF-1α expression with patient survival was evaluated by using the Kaplan-Meier approach and Cox proportional-hazards regression analysis. RESULTS: Among our study population, 56 patients (63.6%) had high levels of HIF-1α expression. High expression of HIF-1α was associated with high expression of MIB-1 (P = 0.04), CD31 (P = 0.03), and VEGF-A (P < 0.0001), as well as necrosis (P = 0.04). However, high HIF-1α expression was not correlated with cell type, largest macroscopic tumor dimension or thickness, anterior margin, pigmentation, or mitotic figures. Patients with high HIF-1α expression did not show a reduced survival when compared to patients with low HIF-1α expression (P = 0.92). Finally, HIF-1α expression was not increased after irradiation. CONCLUSIONS: An increase in HIF-1α expression was significantly associated with proliferative (MIB-1) and vascular (CD31 and VEGF-A) markers, as well as necrosis, in UM. However, there was no correlation between high HIF-1α expression and patient survival.

4 Article Altered expression of the poly(ADP-ribosyl)ation enzymes in uveal melanoma and regulation of PARG gene expression by the transcription factor ERM. 2012

Molloy-Simard, Vanessa / St-Laurent, Jean-François / Vigneault, François / Gaudreault, Manon / Dargis, Natasha / Guérin, Marie-Christine / Leclerc, Steeve / Morcos, Mohib / Black, Daniel / Molgat, Yvonne / Bergeron, Dan / de Launoit, Yvan / Boudreau, François / Desnoyers, Serge / Guérin, Sylvain. ·LOEX/CUO-Recherche, Hôpital du Saint-Sacrement, Centre de recherche du CHA, Québec, Canada. ·Invest Ophthalmol Vis Sci · Pubmed #22915039.

ABSTRACT: PURPOSE: Poly(ADP-ribosyl)ation is a reversible post-translational modification that requires the contribution of the enzymes poly(ADP-ribose) polymerase-1 (PARP-1) and poly(ADP-ribose) glycohydrolase (PARG). Our study explores expression and activity of PARP-1 and PARG in uveal melanoma cell lines with varying tumorigenic properties. METHODS: Gene profiling on microarrays was conducted using RNA prepared from the uveal melanoma cell lines T97, T98, T108, and T115. The activity of PARP-1 and PARG was monitored by enzymatic assays, whereas their expression was measured by Western blot and PCR. The PARG promoter was analyzed using promoter deletions and site-specific mutagenesis in transfection analyses. The transcription factors binding the PARG promoter were studied by electrophoretic mobility shift assay (EMSA) analyses. Suppression of PARP-1 and PARG expression was performed in T97 and T115 cells by RNAi, and their tumorigenic properties monitored by injections into athymic mice. RESULTS: Expression of PARP-1 was found to vary considerably between uveal melanoma cell lines with distinctive tumorigenic properties in vivo. Sp1 and the ETS protein ERM were shown to bind to the PARG gene promoter to ensure basal transcription in uveal melanoma. Importantly, suppression of PARG gene expression in T97 and T115 cells increased their capacity to form tumors in athymic mice, whereas suppression of PARP-1 significantly reduced or almost entirely abolished tumor formation. CONCLUSIONS: Our results suggest that while overexpression of PARP-1 may confer a proliferative advantage to aggressive uveal melanoma tumors, PARG may, on the other hand, support a tumor suppressor function in vivo.

5 Article Suppression of α5 gene expression is closely related to the tumorigenic properties of uveal melanoma cell lines. 2011

Landreville, Solange / Vigneault, François / Bergeron, Marjorie-Allison / Leclerc, Steeve / Gaudreault, Manon / Morcos, Mohib / Mouriaux, Frédéric / Salesse, Christian / Guérin, Sylvain L. ·Department of Ophthalmology & Visual Sciences, Washington University School of Medicine, St. Louis, MO, USA. ·Pigment Cell Melanoma Res · Pubmed #21592318.

ABSTRACT: Cancer aggressiveness is related to the ability of cancer cells to escape the anchorage dependency toward the extracellular matrix, a process regulated by the integrin α5β1 and its ligand fibronectin. Here, we characterized the expression of the α5 gene in human uveal melanoma cell lines with distinct tumorigenic properties and investigated some of the mechanisms underlying the variations of their malignancy. Strong and weak expression of α5 was observed in cells with no (T108/T115) and high (T97/T98) tumorigenic properties, respectively. Expression and DNA binding of the transcription factors Sp1, activator protein 1 (AP-1) (both acting as activators), and nuclear factor I (NFI) (a strong repressor) to the α5 promoter were demonstrated in all cell lines. A reduced expression of AP-1 combined with a dramatic increase in NFI correlated with the suppression of α5 expression in T97 and T98 cells. Restoring α5 expression in T97 cells entirely abolished their tumorigenicity in immunodeficient mice. These uveal melanoma cell lines might therefore prove particularly useful as cellular models to investigate α5β1 function in the pathogenesis of invasive uveal melanoma.