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Melanoma: HELP
Articles by Arindam Sen
Based on 2 articles published since 2010
(Why 2 articles?)
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Between 2010 and 2020, Arindam Sen wrote the following 2 articles about Melanoma.
 
+ Citations + Abstracts
1 Article Metabolic reprogramming of stromal fibroblasts by melanoma exosome microRNA favours a pre-metastatic microenvironment. 2018

Shu, Shin La / Yang, Yunchen / Allen, Cheryl L / Maguire, Orla / Minderman, Hans / Sen, Arindam / Ciesielski, Michael J / Collins, Katherine A / Bush, Peter J / Singh, Prashant / Wang, Xue / Morgan, Martin / Qu, Jun / Bankert, Richard B / Whiteside, Theresa L / Wu, Yun / Ernstoff, Marc S. ·Department of Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. · Department of Biomedical Engineering, Jacobs School of Medicine & Biomedical Sciences, University at Buffalo, The State University of New York, Buffalo, NY, USA. · Flow and Image Cytometry Shared Resource, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. · Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. · Department of Neurosurgery, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. · Immune Analysis Facility, Center for Immunotherapy, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. · South Campus Instrumentation Center, University at Buffalo, The State University of New York, Buffalo, NY, USA. · Genomics Shared Resource, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. · New York Center of Excellence in Bioinformatics and Life Sciences, Buffalo, NY, USA. · Department of Biostatistics and Bioinformatics, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. · Department of Microbiology and Immunology, Jacobs School of Medicine & Biomedical Sciences, University at Buffalo, The State University of New York, Buffalo, NY, USA. · Department of Pathology, Immunology and Otolaryngology, University of Pittsburgh School of Medicine and UPMC Hillman Cancer Center, Pittsburgh, PA, USA. · Department of Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. Marc.Ernstoff@RoswellPark.org. ·Sci Rep · Pubmed #30150674.

ABSTRACT: Local acidification of stroma is proposed to favour pre-metastatic niche formation but the mechanism of initiation is unclear. We investigated whether Human Melanoma-derived exosomes (HMEX) could reprogram human adult dermal fibroblasts (HADF) and cause extracellular acidification. HMEX were isolated from supernatants of six melanoma cell lines (3 BRAF V600E mutant cell lines and 3 BRAF wild-type cell lines) using ultracentrifugation or Size Exclusion Chromatography (SEC). Rapid uptake of exosomes by HADF was demonstrated following 18 hours co-incubation. Exposure of HDAF to HMEX leads to an increase in aerobic glycolysis and decrease in oxidative phosphorylation (OXPHOS) in HADF, consequently increasing extracellular acidification. Using a novel immuno-biochip, exosomal miR-155 and miR-210 were detected in HMEX. These miRNAs were present in HMEX from all six melanoma cell lines and were instrumental in promoting glycolysis and inhibiting OXPHOS in tumour cells. Inhibition of miR-155 and miR-210 activity by transfection of miRNA inhibitors into HMEX reversed the exosome-induced metabolic reprogramming of HADF. The data indicate that melanoma-derived exosomes modulate stromal cell metabolism and may contribute to the creation of a pre-metastatic niche that promotes the development of metastasis.

2 Article Mild elevation of body temperature reduces tumor interstitial fluid pressure and hypoxia and enhances efficacy of radiotherapy in murine tumor models. 2011

Sen, Arindam / Capitano, Maegan L / Spernyak, Joseph A / Schueckler, John T / Thomas, Seneca / Singh, Anurag K / Evans, Sharon S / Hylander, Bonnie L / Repasky, Elizabeth A. ·Department of Immunology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA. ·Cancer Res · Pubmed #21512134.

ABSTRACT: Human and rodent solid tumors often exhibit elevated interstitial fluid pressure (IFP). This condition is recognized as a prognostic indicator for reduced responses to therapy and decreased disease-free survival rate. In the present study, we tested whether induction of a thermoregulatory-mediated increase in tissue blood flow, induced by exposure of mice to mild environmental heat stress, could influence IFP and other vascular parameters within tumors. Using several murine tumor models, we found that heating results in a sustained reduction in tumor IFP correlating with increased tumor vascular perfusion (measured by fluorescent imaging of perfused vessels, laser Doppler flowmetry, and MRI) as well as a sustained reduction in tumor hypoxia. Furthermore, when radiation therapy was administered 24 hours postheating, we observed a significant improvement in efficacy that may be a result of the sustained reduction in tumor hypoxia. These data suggest, for the first time, that environmental manipulation of normal vasomotor function is capable of achieving therapeutically beneficial changes in IFP and microvascular function in the tumor microenvironment.