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Melanoma: HELP
Articles from Taiwan
Based on 327 articles published since 2009
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These are the 327 published articles about Melanoma that originated from Taiwan during 2009-2019.
 
+ Citations + Abstracts
Pages: 1 · 2 · 3 · 4 · 5 · 6 · 7 · 8 · 9 · 10 · 11 · 12 · 13 · 14
1 Editorial Acral melanoma: a unique disease in Asia. 2013

Chang, John Wen-Cheng. ·Division of Hematology-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan, Taiwan. ·JAMA Dermatol · Pubmed #24068331.

ABSTRACT: -- No abstract --

2 Review Primary Vaginal Melanoma With Rhabdoid Features: A Case Report and Literature Review. 2017

Lee, Chien-Kuan / Lin, Ho / Su, Chi-Feng / Kok, Victor C. ·Departments of Pathology (C.-K.L., H.L.)Obstetrics & Gynecology (C.-F.S.)Division of Medical Oncology (V.C.K.), Kung Tien General Hospital, Shalu, Taichung, Taiwan. ·Int J Gynecol Pathol · Pubmed #28800579.

ABSTRACT: Primary vaginal melanoma is a rare mucosal neoplasm, which is more aggressive than cutaneous melanoma. Information regarding its morphologic patterns is limited. In particular, the rhabdoid phenotype, mostly observed in metastatic or recurrent cutaneous melanomas, has yet to be reported at this anatomic location. Hence, a potential diagnostic difficulty may arise because of the inability to recognize this unusual histologic variant and its immunohistochemical aberrance. In this report, we describe the case of a primary vaginal melanoma in a 62-year-old woman, who exhibited both rhabdoid and small blue round cell morphologies, absence of S100 protein, and aberrant expression of desmin, CD56, and FLI-1. This report can facilitate the task of expanding the morphologic spectrum of vaginal melanoma, and prevent misdiagnosis and inadequate medical treatment.

3 Review Recent Advances in Polymeric Nanosystems for Treating Cutaneous Melanoma and Its Metastasis. 2017

Chou, Yi-Ping / Lin, Yin-Ku / Chen, Chun-Han / Fang, Jia-You. ·Division of Trauma, Department of Emergency, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. · Research Center for Industry of Human Ecology and Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, Kweishan, Taoyuan, Taiwan. · School of Traditional Chinese Medicine, Chang Gung University, Kweishan, Taoyuan, Taiwan. · Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital at Keelung, Keelung, Taiwan. · Division of General Surgery, Department of Surgery, Chang Gung Memorial Hospital at Chiayi, Chiayi, Taiwan. · Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Kweishan, Taoyuan, Taiwan. · Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Kweishan, Taoyuan, Taiwan. · Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Kweishan, Taoyuan, Taiwan. · Department of Anesthesiology, Chang Gung Memorial Hospital at Linkou, Kweishan, Taoyuan, Taiwan. ·Curr Pharm Des · Pubmed #28699537.

ABSTRACT: Melanoma shows a high possibility of mortality after it metastasizes because of its aggressive nature. Although there are several options for anti-melanoma therapy, this skin malignancy is resistant to some therapies. Chemotherapy, biochemotherapy, immunotherapy, and adoptive cell therapy have failed to exhibit a significant amelioration in overall survival. Nanomedicine provides an opportunity to improve the efficiency of the antimelanoma regimen. Nanoparticles for treating melanoma provide the advantages over conventional therapies such as drug solubility increment, drug stability enhancement, epithelium permeability and bioavailability amelioration, half-life prolonging, tumor targeting, and side effect minimization. Polymeric nanocarriers are the most extensively studied platforms for the treatment of a variety of cancers. The polymers' sophisticated material engineering tailors the controllable physicochemical properties of the nanoparticles for melanoma penetration via passive and active delivery. The present study highlights the recent progress on the development of polymeric nanoparticles for melanoma treatment. We describe the concepts and improvement mechanisms of the nanomedical techniques for melanoma treatment. Passive targeting by modifying the structure and physicochemical characters of polymeric nanocarriers is a strategy for efficient drug delivery to the melanoma and its metastasis. On the other hand, active targeting such as peptide or antibody conjugation is another approach delivering the drugs or genes to the nidus site by the nanocarriers. This review offers an overview of the benefits of polymeric nanosystems for treating melanoma.

4 Review Tumor cell vascular mimicry: Novel targeting opportunity in melanoma. 2016

Hendrix, Mary J C / Seftor, Elisabeth A / Seftor, Richard E B / Chao, Jun-Tzu / Chien, Du-Shieng / Chu, Yi-Wen. ·Stanley Manne Children's Research Institute, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, IL 60614, United States; Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, United States. Electronic address: m-hendrix@northwestern.edu. · Stanley Manne Children's Research Institute, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, IL 60614, United States. · Stanley Manne Children's Research Institute, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, IL 60614, United States; Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, United States. · TaiRx, Inc., Taipei, 11560, Taiwan. ·Pharmacol Ther · Pubmed #26808163.

ABSTRACT: In 1999, the American Journal of Pathology published an article, entitled "Vascular channel formation by human melanoma cells in vivo and in vitro: vasculogenic mimicry" by Maniotis and colleagues, which ignited a spirited debate for several years and earned the journal's distinction of a "citation classic" (Maniotis et al., 1999). Tumor cell vasculogenic mimicry (VM), also known as vascular mimicry, describes the plasticity of aggressive cancer cells forming de novo vascular networks and is associated with the malignant phenotype and poor clinical outcome. The tumor cells capable of VM share the commonality of a stem cell-like, transendothelial phenotype, which may be induced by hypoxia. Since its introduction as a novel paradigm for melanoma tumor perfusion, many studies have contributed new findings illuminating the underlying molecular pathways supporting VM in a variety of tumors, including carcinomas, sarcomas, glioblastomas, astrocytomas, and melanomas. Of special significance is the lack of effectiveness of angiogenesis inhibitors on tumor cell VM, suggesting a selective resistance by this phenotype to conventional therapy. Facilitating the functional plasticity of tumor cell VM are key proteins associated with vascular, stem cell, extracellular matrix, and hypoxia-related signaling pathways--each deserving serious consideration as potential therapeutic targets and diagnostic indicators of the aggressive, metastatic phenotype. This review highlights seminal findings pertinent to VM, including the effects of a novel, small molecular compound, CVM-1118, currently under clinical development to target VM, and illuminates important molecular pathways involved in the suppression of this plastic, aggressive phenotype, using melanoma as a model.

5 Review Posterior choroidal leiomyoma: a rare case report and literature review. 2015

Chiang, Wei-Yu / Lin, Jui-Wei / Yang, I-Hui / Kuo, Hsi-Kung. ·Department of Ophthalmology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan. · Department of Pathology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan. ·APMIS · Pubmed #25907891.

ABSTRACT: We report a literature review and detailed evaluation of a rare case of posterior choroidal leiomyoma to emphasize the importance of differentiating this from other choroidal tumors. A 30-year-old male presented with variable blurred vision in his right eye secondary to a choroidal tumor. Clinical examinations were performed including fundus photography, optical coherence tomography, B scans, fluorescein and indocyanine green angiography, computed tomography, and magnetic resonance imaging. Preoperative examination revealed a suspected choroidal melanoma and enucleation was performed. However, a definitive diagnosis of choroidal leiomyoma was made following postoperative pathological light microscopy and immunohistochemical studies. Published case reports were collected and the common characteristics and distinctive features were compared with the current case. Posterior choroidal leiomyoma was summarized from the literature, and beneficial information for diagnosis and treatment was obtained. In conclusion, posterior choroidal leiomyoma is rare and should be differentiated from amelanotic melanomas. Despite the benign nature, an explanation regarding the rare incidence and difficult diagnosis of posterior choroidal leiomyoma must be provided to patients, prior to enucleation or detrimental treatment.

6 Review What are the potential benefits of using proton therapy in Taiwanese cancer patients? 2015

Kao, Wei-Heng / Shen, Yi-Liang / Hong, Ji-Hong. ·Department of Radiation Oncology, Chang Gung Memorial Hospital at Linkou, Chang Gung University College of Medicine; Institute for Radiological Research, Chang Gung University/Chang Gung Memorial Hospital; Department of Medical Imaging and Radiological Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan. ·Biomed J · Pubmed #25768324.

ABSTRACT: The potential benefits of proton therapy have been established in pediatric cancer, skull base tumor, uveal melanoma, and other types of cancers. Western and Asian countries, however, have differences in the pattern of cancer incidence; this leads to the difference in patient demographics for proton therapy. Furthermore, the advancement of the scanning beam technique in proton therapy greatly expands the capability of proton therapy in disease sites with great complexity. In this review, we focus on the cancers with high incidence in Taiwan, based on the Cancer Registry Annual Report, 2011, Taiwan. The potential case number and clinical benefits from proton therapy are evaluated and discussed. Two endemic cancers, hepatocellular carcinoma and head and neck cancer, are considered to be the major disease types appropriate for proton therapy in Taiwan. Primary lung cancer and left side breast cancer, which are popular in western countries as well as in Taiwan, are included for discussion. The issue of cost-effectiveness for proton therapy is also reviewed. Finally, we point out the clinical trials that should be conducted for proton therapy in Taiwan.

7 Review Primary leptomeningeal melanoma mimicking meningitis: a case report and literature review. 2015

Hsieh, Yao-Yu / Yang, Shun-Tai / Li, Wei-Hua / Hu, Chaur-Jong / Wang, Liang-Shun. ·Taipei Medical University Shuang-Ho Hospital, New Taipei City, Taiwan. · Taipei Medical University Shuang-Ho Hospital, New Taipei City, Taiwan wangls72269@yahoo.com.tw. ·J Clin Oncol · Pubmed #24637995.

ABSTRACT: -- No abstract --

8 Review Primary leptomeningeal melanoma. 2014

Xie, Zhao-Yu / Hsieh, Kevin Li-Chun / Tsang, Yuk-Ming / Cheung, Wing-Keung / Hsieh, Chen-Hsi. ·Department of Radiology, Division of Medical Imaging, Far Eastern Memorial Hospital, Number 21, Section 2, Nanya S. Road, Banqiao District, New Taipei City 220, Taiwan. · Department of Radiology, Division of Medical Imaging, Far Eastern Memorial Hospital, Number 21, Section 2, Nanya S. Road, Banqiao District, New Taipei City 220, Taiwan. Electronic address: kevinh9396@gmail.com. · Division of Radiation Oncology, Department of Radiology, Far-Eastern Memorial Hospital, New Taipei City, Taiwan. ·J Clin Neurosci · Pubmed #24331626.

ABSTRACT: Primary melanoma of the central nervous system is a rare melanocytic tumor typically located in the leptomeninges. We report a 57-year-old woman with an intracranial leptomeningeal melanoma who presented with myoclonic seizures. Brain CT scan and MRI revealed a hemorrhagic intracranial tumor. The tumor was completely removed and leptomeningeal melanoma was proven pathologically. Follow-up imaging studies up to 19 months showed no recurrence of the disease. Here we present radiological, gross, and pathological images of leptomeningeal melanoma, discuss its characteristics, and review the relevant literature.

9 Review Molecular mechanisms of garlic-derived allyl sulfides in the inhibition of skin cancer progression. 2012

Wang, Hsiao-Chi / Pao, Jung / Lin, Shuw-Yuan / Sheen, Lee-Yan. ·Institute of Food Science and Technology, National Taiwan University, Taipei, ROC. ·Ann N Y Acad Sci · Pubmed #23050963.

ABSTRACT: Skin cancer is a serious concern whose incidence is increasing at an alarming rate. Allyl sulfides-i.e., sulfur metabolites in garlic oil-have been demonstrated to have anticancer activity against several cancer types, although the mechanisms underlying these effects remain enigmatic. Our previous study showed that diallyl trisulfide (DATS) is more potent than mono- and disulfides against skin cancer. DATS inhibits cell growth of human melanoma A375 cells and basal cell carcinoma (BCC) cells by increasing the levels of intracellular reactive oxygen species (ROS) and DNA damage and by inducing G2/M arrest, endoplasmic reticulum (ER) stress, and mitochondria-mediated apoptosis, including the caspase-dependent and -independent pathways. This short review focuses on the molecular mechanisms of garlic-derived allyl sulfides on skin cancer prevention.

10 Review Cutaneous melanoma: Taiwan experience and literature review. 2010

Chang, John Wen-Cheng. ·Division of Hematology-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Chang Gung University College of Medicine, Taoyuan, Taiwan, ROC. wen1902@hotmail.com ·Chang Gung Med J · Pubmed #21199605.

ABSTRACT: Malignant melanoma is a rare disease in Taiwan with an incidence rate of 0.65/100,000. Excessive exposure to ultraviolet radiation is not associated with most Taiwanese melanoma cases. Acral lentiginous melanoma comprises 58% of cutaneous melanoma. Advanced disease is seen in 50% of cases. Surgery, including resection of the primary melanoma, sentinel lymph nodes that may harbor microscopic metastasis, clinically abnormal lymph nodes, and selected distant metastases, is the most important treatment. Lymphatic mapping and sentinel lymph node biopsy has changed the clinical stage in 22.2% of our patients. Adjuvant high-dose interferon significantly prolongs progression-free survival. However, its use in Taiwan is limited by its substantial toxicity. The prognosis of metastatic disease remains poor with a median survival of 12 months. In the past, chemotherapy alone was the most common treatment modality for metastatic disease. Recently biochemotherapy has been more commonly utilized to treat patients with metastatic melanoma.

11 Clinical Trial Immunotherapy with dendritic cells pulsed by autologous dactinomycin-induced melanoma apoptotic bodies for patients with malignant melanoma. 2009

Chang, John W C / Hsieh, Jia-Juan / Shen, Yung-Chi / Ho, E / Chuang, Cheng-Keng / Chen, Yu-Ray / Liao, Shuen-Kuei / Chen, Jen-Shi / Leong, Stanley P L / Hou, Ming-Mo / Chang, Nai-Jen / Wang, Cheng-Hsu. ·Division of Hematology-Oncology, Department of Internal Medicine, Pathology, Chang Gung Memorial Hospital, Taoyuan, Taiwan. wen1902@hotmail.com ·Melanoma Res · Pubmed #19750589.

ABSTRACT: The primary goal of this study was to evaluate the efficacy of immunotherapy for patients with metastatic melanoma with autologous melanoma apoptotic bodies (MAB)-pulsed dendritic cells (DCs). Accessible tumors from eligible patients with refractory metastatic melanoma were surgically removed and processed for primary culture. The autologous tumor cells were treated with dactinomycin to obtain MAB. To generate DCs, adherent peripheral blood mononuclear cells were cultured in complete medium containing granulocyte macrophage-colony stimulating factor and interleukin-4. MAB-pulsed DCs were given either intradermally (i.d.) or intravenously. Patients were immunized at monthly intervals and boosted with keyhole limpet hemocyanin (KLH) and MAB 2 weeks post-vaccination, with a maximum of four cycles. Of the 10 patients enrolled in this trial, nine were treated with MAB-pulsed DCs; two were given intravenous vaccinations and the other seven were i.d. injected. Mild tenderness in the draining lymph nodes lasting for less than 48 h and enlargement of the draining lymph nodes were noted in all seven i.d. cases. Treatment-related grade 3-4 toxicity, neutropenia, skin ulceration, tumor growth at the injection site, and sepsis were not observed in any of the patients. Delayed-type hypersensitivity to KLH was observed in all patients, whereas no delayed-type hypersensitivity to autologous tumor antigens was observed. One patient achieved partial response with reduction in lung metastatic tumor mass, and a presence of vesicles in the post-vaccination KLH response. Two patients had stable disease for more than 24 months; one was still alive at the time of submission of this report, the other eventually developed multiple metastases. MAB-pulsed DC immunotherapy is well tolerated in patients with malignant melanoma; however, its efficacy is only modest. Combination with other modalities is required to enhance DC-based immunotherapy.

12 Article Scalp melanoma with rectus abdominis metastasis: A rare case report. 2019

Hsu, Kuo-Feng / Chen, Chun-Yu / Chu, Tzi-Shiang / Liu, Hung-Hui / Chang, Chun-Kai / Wu, Chien-Ju / Lin, Chin-Ta / Wang, Chih-Hsin / Tzeng, Yuan-Sheng / Dai, Niann-Tzyy / Chen, Shyi-Gen. ·Division of Plastic and Reconstructive Surgery, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan. ·Medicine (Baltimore) · Pubmed #31305447.

ABSTRACT: RATIONALE: The main cause of death in melanoma patients is widespread metastases as it can metastasize to almost every organ. However, melanoma skeletal muscle metastases (MSMM) are exceptional, and only a few cases of MSMM to the rectus abdominis muscles have been previously described. And our case maybe the first reported case in Asia region. PATIENT CONCERNS: A 45-year-old man with history of right scalp melanoma, pT3aN0M0, stage IIA status post wide excision with 2 cm safe margin and right neck lymph node dissection at 5 years before. He had an almost 5 years disease-free period but presented to our clinic due to intermittent abdominal sharp pain for 1 to 2 months, with a palpable soft tissue mass over his right abdomen. Metastatic melanoma to rectus abdominis muscles was highly suspected. INTERVENTIONS: The patient subsequently underwent radical en-block extraperitoneal 15 cm segmental resection of the right rectus abdominis muscle including tumor mass. The resected tumor was a black-gray colored solid mass, and the final histologic study showed a metastasis of melanoma. OUTCOMES: Postoperative course of the patient was uneventful, and the right abdominal pain was improved. The patient was referred for further target therapy, but passed away half a year later due to multiple metastasis. LESSONS: Scalp melanoma with isolated rectus muscle metastasis is extremely rare especially for a young aged patient who had an almost 5-year disease-free period. Surgery is a potentially curative therapy for patients with isolated metastatic melanoma. The goal is negative resection margins, in order to avoid local recurrences. Radical compartmental surgery should be considered for selected stage IV melanoma patients with sole rectus abdominis MSMM, whose disease could be amenable to complete resection, in preliminary procedure to prolong disease-free survival time. For oligometastatic disease, surgical resection is sometimes useful in carefully selected patients after systemic therapy; also, it could be performed as symptomatic treatment.

13 Article Structural and functional characterization of β 2019

Leu, Shr-Jeng Jim / Lee, Tzong-Yi / Cheng, Shu-Wei / Tsai, Meng-Ying / Lin, Yu-Shan / Chiou, Tzeon-Jye / Huang, Kai-Yao / Chiang, An-Na. ·Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan. · Department of Education and Research, Taipei City Hospital, Taipei, Taiwan. · Warshel Institute for Computational Biology, Chinese University of Hong Kong, Shenzhen, China. · Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan. · Division of Transfusion Medicine, Taipei Veterans General Hospital, and School of Medicine, National Yang-Ming University, Taipei, Taiwan. ·Cancer Sci · Pubmed #31012976.

ABSTRACT: We previously found that circulating β

14 Article Effects of extremely low-frequency electromagnetic fields on B16F10 cancer cells. 2019

Tang, Jing-Yau / Yeh, Te-Wei / Huang, Yu-Ting / Wang, Min-Haw / Jang, Ling-Sheng. ·a Department of Electrical Engineering , National Cheng Kung University , Tainan , Taiwan. · b Department of Electrical Engineering , Chinese Culture University , Taipei , Taiwan. ·Electromagn Biol Med · Pubmed #30889982.

ABSTRACT: This paper presents a method to inhibit B16F10 cancer cells using extremely low-frequency electromagnetic fields (ELF-EMFs) and to evaluate cell viability using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. The study examined the effect of a natural EMF resonance frequency (7.83 Hz) and a power line frequency (60 Hz) on B16F10 cancer cells for 24 and 48 h. The B16F10 cancer cells were also exposed to sweep frequencies in several sweep intervals to quantitatively analyze the viability of cancer cells. The results yielded a 17% inhibition rate under 7.83 Hz compared with that of the control group. Moreover, sweep frequencies in narrow intervals (7.83 ± 0.1 Hz for the step 0.05 Hz) caused an inhibition rate of 26.4%, and inhibitory effects decreased as frequency sweep intervals increased. These results indicate that a Schumann resonance frequency of 7.83 Hz can inhibit the growth of cancer cells and that using a specific frequency type can lead to more effective growth inhibition.

15 Article Preparation and Evaluation of Novel Transfersomes Combined with the Natural Antioxidant Resveratrol. 2019

Wu, Pey-Shiuan / Li, Yu-Syuan / Kuo, Yi-Ching / Tsai, Suh-Jen Jane / Lin, Chih-Chien. ·Department of Cosmetic Science, Providence University, Taichung 43301, Taiwan. jwu2@pu.edu.tw. · Department of Cosmetic Science, Providence University, Taichung 43301, Taiwan. g1060037@gm.pu.edu.tw. · Department of Cosmetic Science, Providence University, Taichung 43301, Taiwan. sin14990@gmail.com. · Department of Applied Chemistry, Providence University, Taichung 43301, Taiwan. sjtsai@pu.edu.tw. · Department of Cosmetic Science, Providence University, Taichung 43301, Taiwan. chchlin@pu.edu.tw. ·Molecules · Pubmed #30743989.

ABSTRACT: Resveratrol (

16 Article Photosensitizer-conjugated Cu-In-S heterostructured nanorods for cancer targeted photothermal/photodynamic synergistic therapy. 2019

Chen, Si-Han / Huang, Wan-Wen / Dehvari, Khalilalrahman / Ling, Yong-Chien / Ghule, Anil V / Tsai, Shen-Long / Chang, Jia-Yaw. ·Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan, Republic of China. · Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan, Republic of China. · Department of Chemistry, Shivaji University, Kolhapur 416004, Maharashtra, India. Electronic address: anighule@gmail.com. · Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan, Republic of China. Electronic address: stsai@mail.ntust.edu.tw. · Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan, Republic of China; Taiwan Building Technology Center, National Taiwan University of Science and Technology, Taipei 10607, Taiwan, Republic of China. Electronic address: jychang@mail.ntust.edu.tw. ·Mater Sci Eng C Mater Biol Appl · Pubmed #30678970.

ABSTRACT: Photo-activated therapy is a non-invasive and promising medical technology for the treatment of cancers. Herein, we present Ce6-HA-CIS phototherapeutic nanohybrids composed of Cu-In-S (CIS) heterostructured nanorod (HS-rod), chlorin e6 (Ce6), and hyaluronic acid (HA) for the use in targeted photodynamic/photothermal therapy (PDT/PTT). In the Ce6-HA-CIS nanohybrids, the CIS HS-rod was investigated as a PTT agent to convert light into thermal energy, with Ce6 acting as a PDT agent to generate singlet oxygen (

17 Article Phyto-sesquiterpene lactone deoxyelephantopin and cisplatin synergistically suppress lung metastasis of B16 melanoma in mice with reduced nephrotoxicity. 2019

Chao, Wen-Wan / Cheng, Ya-Wen / Chen, Yet-Ran / Lee, Shu-Hua / Chiou, Ching-Yi / Shyur, Lie-Fen. ·Department of Nutrition and Health Sciences, Kainan University, Taoyuan 338, Taiwan. · Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan. · Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan; PhD Program in Translational Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei 110, Taiwan. Electronic address: lfshyur@ccvax.sinica.edu.tw. ·Phytomedicine · Pubmed #30668340.

ABSTRACT: BACKGROUND: Cisplatin (CP) is a chemotherapeutic drug for treating melanoma that also causes adverse side effects in cancer patients. PURPOSE: This study investigated the bioefficacy of a phytoagent deoxyelephantopin (DET) in inhibiting B16 melanoma cell activity, its synergism with CP against metastatic melanoma, and its capability to attenuate CP side effects in animals. METHODS: DET and CP bioactivities were assessed by MTT assay, isobologram analysis, time-lapse microscopy, migration and invasion assays, flow cytometry and western blotting. In vivo bioluminescence imaging was used to detect lung metastasis of B16 cells carrying COX-2 reporter gene in syngeneic mice. H&E staining and immunohistochemistry were used to evaluate the compound/drug efficacy and CP side effects. Nephrotoxicity caused by CP treatment in mice was evaluated by UPLC/ESI-QTOF MS - based metabolomics and haematometry. RESULT: DET, alone or in combination with cisplatin, inhibited B16 cell proliferation, migration, and invasion, and induced cell-cycle arrested at the G CONCLUSION: The CP and DET combination may be an effective intervention for melanoma with reduced side effects.

18 Article Anti-melanization effects and inhibitory kinetics of tyrosinase of bird's nest fern (Asplenium australasicum) frond extracts on melanoma and human skin. 2019

Zeng, Wen-Wen / Lai, Lih-Shiuh. ·Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Rd, Taichung 40227, Taiwan. · Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Rd, Taichung 40227, Taiwan. Electronic address: lslai@dragon.nchu.edu.tw. ·J Biosci Bioeng · Pubmed #30639118.

ABSTRACT: Some bioactive properties of p-coumaric acid and fucose-rich polysaccharide in skin health have been studied, including melanogenesis inhibition of the phenolic acid and growth inhibitory effects of the polysaccharide on melanoma. The dermatological benefits of bird's nest fern extracts (BNFE), containing both substantial fucose-rich polysaccharide and p-coumaric acid, like promoting collagen production and growth of fibroblast cell and further improving the elasticity and dryness of human skins have been demonstrated in our previous study. Besides, the anti-melanization effects of various BNFE on B16-F10 melanoma and human skin were first studied here. The promising extracts revealed that the main phenolic acid, p-coumaric acid, in BNFE resulted in suppression against tyrosinase activity from melanogenesis. The inhibitory kinetics on the diphenolase activity indicated that AE40 was a noncompetitive inhibitor of mushroom tyrosinase. On the other hand, the fucose-rich mucilage of BNFE showed pronouncedly suppressing effect on B16-F10 melanoma viability. Clinical trial was performed by recruiting 46 female volunteers and the results indicated that the lotions with 1% of BNFE was non-irritant and reduced effectively the pigmentation on human skin after 7-14 days of continuous application. It was suggested that the fucose-rich mucilage and p-coumaric acid in BNFE may have potential for nutricosmetics and phytotherapy applications as a natural hypopigmenting agent.

19 Article Chrysin inhibit human melanoma A375.S2 cell migration and invasion via affecting MAPK signaling and NF-κB signaling pathway in vitro. 2019

Chen, Hsin-Yu / Jiang, Yi-Wen / Kuo, Chao-Lin / Way, Tzong-Der / Chou, Yu-Cheng / Chang, Yuan-Shiun / Chung, Jing-Gung. ·Department of Biological Science and Technology, China Medical University, Taichung, Taiwan. · Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan. · Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung, Taiwan. · Department of Neurological Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan. · Department of Biotechnology, Asia University, Taichung, Taiwan. ·Environ Toxicol · Pubmed #30578657.

ABSTRACT: Numerous evidences have shown that chrysin induced cytotoxic effects via induced cell cycle arrest and induction of cell apoptosis in human cancer cell lines, however, no information showed that chrysin inhibited skin cancer cell migration and invasion. In this study, we investigated anti-metastasis mechanisms of chrysin in human melanoma cancer A375.S2 cells in vitro. Under sub-lethal concentrations of chrysin (0, 5, 10, and 15 μM) which inhibits cell mobility, migration and invasion of A375.S2 cells that were assayed by wound healing and Transwell filter. That chrysin inhibited MMP-2 activity in A375.S2 cells was investigated by gelatin zymography assay. Western blotting was used to examine protein expression and results indicated that chrysin inhibited the expression of GRB2, SOS-1, PKC, p-AKT (Thr308), NF-κBp65, and NF-κBp50 at 24 and 48 hours treatment, but only at 10-15 μM of chrysin decreased Ras, PI3K, p-c-Jun, and Snail only at 48 hours treatment and only decrease p-AKT(Ser473) at 24 hours treatment. Furthermore, chrysin (5-15 μM) decreased the expression of uPA, N-cadherin and MMP-1 at 24 and 48 hours treatment but only decreased MMP-2 and VEGF at 48 hours treatment at 10-15 μM and 5-15 μM of chrysin, respectively, however, increased E-cadherin at 5-15 μM treatment. Results of confocal laser microscopy systems indicated that chrysin inhibited expression of NF-κBp65 in A375.S2 cells. Based on these observations, we suggest that chrysin can be used in anti-metastasis of human melanoma cells in the future.

20 Article Safety assessment, biological effects, and mechanisms of Myrica rubra fruit extract for anti-melanogenesis, anti-oxidation, and free radical scavenging abilities on melanoma cells. 2019

Juang, Lih-Jeng / Gao, Xiang-Yu / Mai, Shou-Ting / Lee, Cheng-Hung / Lee, Ming-Chung / Yao, Chao-Ling. ·Graduate School of Health Industry Management, Ching Kuo Institute of Management and Health, Keelung, Taiwan. · Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan City, Taiwan. · Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan City, Taiwan. · Department of General Surgery, Buddhist Dalin Tzu Chi Hospital, Chia-Yi, Taiwan. · School of Medicine, Tzu Chi University, Hualien, Taiwan. · Brion Research Institute of Taiwan, New Taipei City, Taiwan. ·J Cosmet Dermatol · Pubmed #29460390.

ABSTRACT: OBJECTIVE: Currently, the cosmetic and medical industries are paying considerable attention to solve or prevent skin damage or diseases, such as hyperpigmentation and oxidation and free radical damage. In this study, the effective compounds in Myrica rubra fruit were extracted and studied the biological effects of these M. rubra fruit extracts. METHODS: In this study, we extracted M. rubra fruit using solutions with various ratios of water to ethanol (100:0, 50:50, 5:95) and studied the anti-melanogenesis, anti-oxidation and radical scavenging effects of these M. rubra fruit extracts on two melanoma cell lines: mouse melanoma (B16-F0) and human melanoma (A2058). The cytotoxicity, melanin synthesis, mushroom and cellular tyrosinase activities, enzyme kinetics, melanogenesis-related gene expression, melanogenesis-related protein secretion, radical DPPH scavenging activity and ROS inhibition after treatment with M. rubra fruit extracts were determined. RESULTS: The results showed that the water extract of M. rubra fruit was less cytotoxic to the melanoma cell lines, effectively inhibited melanin synthesis and tyrosinase activity and down-regulated the gene expression and protein secretion of MITF and TRP-1. In addition, the M. rubra fruit extracts also showed the abilities to scavenge DPPH free radicals and suppress ROS production. Finally, the effective compounds in the water extract were Myricetin-O-deoxyhexoside, Quercetin-O-deoxyhexoside, and Kaempferol-O-hexoside determined by LC/MS/MS assay. CONCLUSION: Overall, the water extract of M. rubra fruit is a safe and effective melanin inhibitor and anti-oxidant and can be applied widely in the fields of cosmetics and medicine.

21 Article Melanogenesis Inhibitors from the Rhizoma of Ligusticum Sinense in B16-F10 Melanoma Cells In Vitro and Zebrafish In Vivo. 2018

Cheng, Min-Chi / Lee, Tzong-Huei / Chu, Yi-Tzu / Syu, Li-Ling / Hsu, Su-Jung / Cheng, Chia-Hsiung / Wu, Jender / Lee, Ching-Kuo. ·School of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan. d301100008@tmu.edu.tw. · Institute of Fisheries Science, National Taiwan University, Taipei 10617, Taiwan. thlee1@ntu.edu.tw. · Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei 11031, Taiwan. m303098006@tmu.edu.tw. · Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei 11031, Taiwan. goat1201@hotmail.com. · Department of Food Science, National Chiayi University, Chiayi 60004, Taiwan. r1101815@yahoo.com.tw. · Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan. chcheng@tmu.edu.tw. · School of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan. cklee@tmu.edu.tw. · School of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan. jd0332@tmu.edu.tw. · Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei 11031, Taiwan. jd0332@tmu.edu.tw. · Ph.D. Program in Biotechnology Research and Development, Taipei Medical University, Taipei 11031, Taiwan. jd0332@tmu.edu.tw. ·Int J Mol Sci · Pubmed #30545008.

ABSTRACT: The rhizoma of

22 Article Ganoderma immunomodulatory protein and chidamide down-regulate integrin-related signaling pathway result in migration inhibition and apoptosis induction. 2018

Lu, Chun-Te / Leong, Pui-Ying / Hou, Ting-Yi / Huang, Sheng-Jia / Hsiao, Yu-Ping / Ko, Jiunn-Liang. ·Institute of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Division of Plastic and Reconstructive Surgery, Department of Surgery, Taichung Veterans General Hospital, Taichung, Taiwan. · Institute of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Rheumatology, Chung Shan Medical University Hospital, Taichung 402, Taiwan. · Institute of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan. · Institute of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Dermatology, Chung Shan Medical University Hospital, Taichung, Taiwan. · Institute of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Dermatology, Chung Shan Medical University Hospital, Taichung, Taiwan. Electronic address: missyuping@gmail.com. · Institute of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Medical Oncology and Chest Medicine, Chung Shan Medical University Hospital, Taichung 402, Taiwan. Electronic address: jlko@csmu.edu.tw. ·Phytomedicine · Pubmed #30466626.

ABSTRACT: BACKGROUND: In terms of melanoma, recent advances have been made in target therapies and immune checkpoint inhibitors, but durable remission is rare. Ganoderma immunomodulatory proteins (GMI) induce a cytotoxic effect in cancer cells via autophagy. However, the role of GMI in melanoma is not clear. PURPOSE: The aims of this study are to investigate the inhibiting effects of GMI combined with chidamide on survival and metastases of melanoma cells via integrin-related signaling pathway and to propose strategies for combining GMI and chidamide using animal model. METHODS: Cell viability was measured by cell CCK-8. The activities of apoptosis- and migration-related proteins were detected on Western blot. Flow cytometry was used to analyze cell cycle distribution and sub-G1 fraction in treated melanoma cells. To evaluate the activity of combination GMI and chidamide treatment, an in vivo anti-tumor metastasis study was performed. RESULTS: GMI combined with chidamide additively induced apoptosis. GMI inhibited the expressions of Integrin α5, αV, β1, and β3. The level of p-FAK was inhibited by GMI. Combination treatment of GMI and chidamide decreased survivin and increased cleaved caspase-7 and LC3 II/I. Integrin-αV overexpression activated p-FAK pathways in A375.S2 cells. GMI significantly inhibited cell growth and migration of A375.S2 cells on wound healing assay. In vivo, GMI combined with chidamide suppressed distal tumor metastasis. CONCLUSION: GMI inhibits the migration and growth of melanoma cells via integrin-related signaling pathway. GMI and chidamide induces apoptosis. In vivo, GMI and chidamide additively reduce distant metastases. GMI and chidamide are potential immunotherapeutic adjuvant for metastatic melanoma.

23 Article Distinct MAPK and PI3K pathway mutations in different melanoma types in Taiwanese individuals. 2018

Gao, Hong-Wei / Tsai, Wen-Chiuan / Perng, Cherng-Lih / Wang, Wei-Ming / Chiang, Chien-Ping. ·Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan. · Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Graduate Institute of Biotechnology, Collage of Engineering, National Taipei University of Technology, Taipei, Taiwan. · Division of Clinical Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan. · Department of Dermatology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan. · Department of Dermatology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan. ·Eur J Dermatol · Pubmed #30325319.

ABSTRACT: BACKGROUND: A number of studies investigating mutations of genes involved in MAPK and PI3K pathways in melanoma patients have been performed, most of which were based on Caucasian populations. OBJECTIVES: We sought to identify BRAF, NRAS, MEK1, PI3K, and PTEN mutations and further determine possible correlations with clinicopathological parameters in Taiwanese patients with acral, non-chronic sun damaged (NCSD), or mucosal melanoma. MATERIALS & METHODS: Forty melanocytic nevi, 24 dysplastic nevi, and 175 melanomas from Taiwanese patients were analysed for mutations in BRAF, NRAS, MEK1, PI3K, and PTEN genes by PCR and direct sequencing. Immunohistochemical analysis of the respective proteins in nevi and melanomas were also performed to determine possible clinicopathological characteristics. RESULTS: In addition to the classic BRAF CONCLUSIONS: Our findings suggest that oncogenic events may differ among melanomas in Asian patients geographically (e.g. between Japanese and Taiwanese patients). Moreover, distinct genetic alterations were noted among acral, NCSD, and mucosal melanoma patients in our study, and the expression of biomarkers correlated with clinical survival rates differentially among the various melanoma groups.

24 Article Mechanism of Lakoochin A Inducing Apoptosis of A375.S2 Melanoma Cells through Mitochondrial ROS and MAPKs Pathway. 2018

Peng, Kuo-Ti / Chiang, Yao-Chang / Ko, Horng-Huey / Chi, Pei-Ling / Tsai, Chia-Lan / Ko, Ming-I / Lee, Ming-Hsueh / Hsu, Lee-Fen / Lee, Chiang-Wen. ·Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, Puzi City, Chiayi County 61363, Taiwan. mr3497@adm.cgmh.org.tw. · College of Medicine, Chang Gung University, Guishan Dist., Taoyuan City 33303, Taiwan. mr3497@adm.cgmh.org.tw. · Department of Nursing, Division of Basic Medical Sciences, and Chronic Diseases and Health Promotion Research Center, Chang Gung University of Science and Technology, Puzi City, Chiayi County 61363, Taiwan. yaochang.chiang@gmail.com. · Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. hhko@kmu.edu.tw. · Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Zuoying Dist., Kaohsiung City 81362, Taiwan. chi542738@gmail.com. · Department of Pathology and Laboratory Medicine, Kaohsiung Veterans General Hospital, Zuoying Dist., Kaohsiung City 81362, Taiwan. chi542738@gmail.com. · Department of Nursing, Division of Basic Medical Sciences, Chang Gung University of Science and Technology, Guishan Dist., Taoyuan City 33303, Taiwan. cltsai@mail.cgust.edu.tw. · Department of Nursing, Division of Basic Medical Sciences, and Chronic Diseases and Health Promotion Research Center, Chang Gung University of Science and Technology, Puzi City, Chiayi County 61363, Taiwan. s041055@mail.cgust.edu.tw. · Division of Neurosurgery, Department of Surgery, Chang Gung Memorial Hospital, Puzi City, Chiayi County 61363, Taiwan. ma2072@gmail.com. · Department of Respiratory Care, Chang Gung University of Science and Technology, Puzi City, Chiayi County 61363, Taiwan. ma2072@gmail.com. · Division of Neurosurgery, Department of Surgery, Chang Gung Memorial Hospital, Puzi City, Chiayi County 61363, Taiwan. lfhsu@mail.cgust.edu.tw. · Department of Respiratory Care, Chang Gung University of Science and Technology, Puzi City, Chiayi County 61363, Taiwan. lfhsu@mail.cgust.edu.tw. · Department of Nursing, Division of Basic Medical Sciences, and Chronic Diseases and Health Promotion Research Center, Chang Gung University of Science and Technology, Puzi City, Chiayi County 61363, Taiwan. cwlee@mail.cgust.edu.tw. · Research Center for Industry of Human Ecology and Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, Guishan Dist., Taoyuan City 33303, Taiwan. cwlee@mail.cgust.edu.tw. · Department of Rehabilitation, Chang Gung Memorial Hospital, Chang Gung Memorial Hospital, Puzi City, Chiayi County 61363, Taiwan. cwlee@mail.cgust.edu.tw. ·Int J Mol Sci · Pubmed #30200660.

ABSTRACT: Malignant melanoma is developed from pigment-containing cells, melanocytes, and primarily found on the skin. Malignant melanoma still has a high mortality rate, which may imply a lack of therapeutic agents. Lakoochin A, a compound isolated from

25 Article New diterpenes leojaponins G-L from Leonurus japonicus. 2018

Lai, Kuan-Ying / Hu, Hao-Chun / Chiang, Hsiu-Mei / Liu, Yi-Jung / Yang, Juan-Cheng / Lin, Yen-An / Chen, Chao-Jung / Chang, Yuan-Shiun / Lee, Chia-Lin. ·Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan. · Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. · Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 40447, Taiwan. · Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan. · Graduate Institute of Integrated Medicine, China Medical University, Taichung 40402, Taiwan; Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital, Taichung 40402, Taiwan. · Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, 40402, Taiwan. · Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan; Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 40447, Taiwan; Chinese Medicine Research Center, China Medical University, Taichung 40402, Taiwan. Electronic address: chlilee@mail.cmu.edu.tw. ·Fitoterapia · Pubmed #30149097.

ABSTRACT: Six new diterpenes, leojaponins G-L (1-6) along with 19 known compounds (7-25) were isolated from Leonurus japonicus. Their structures were elucidated by NMR, MS, IR, UV, and ECD spectroscopic data. Anti-melanogenesis assay indicated that 7 could safely and dose-dependently decrease melanin production in B16F10 melanoma cell with an IC

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