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Methicillin-Resistant Staphylococcus aureus: HELP
Articles by Mehmet Kiyan
Based on 3 articles published since 2010
(Why 3 articles?)

Between 2010 and 2020, Mehmet Kıyan wrote the following 3 articles about Methicillin-Resistant Staphylococcus aureus.
+ Citations + Abstracts
1 Article Do Nano-crystalline Silver-Coated Hernia Grafts Reduce Infection? 2018

Nergiz Adıgüzel, Elif / Esen, Ebru / Aylaz, Gökçe / Keskinkılıç Yağız, Betül / Kıyan, Mehmet / Doğan, Aydın / Ünal, Ali Ekrem. ·Department of General Surgery, Lokman Hekim Demet Medical Center, Ankara, Turkey. · Department of General Surgery, Konya Training and Research Hospital, Haci Saban Mah. Meram Yeniyol Caddesi No. 97, 42090, Meram, Konya, Turkey. drebruesen@gmail.com. · Department of General Surgery, Istanbul Health Practice and Research Center, Baskent University, Istanbul, Turkey. · Department of General Surgery, Numune Training and Research Hospital, Ankara, Turkey. · Department of Microbiology, Faculty of Medicine, Ankara University, Ankara, Turkey. · Department of Materials Science and Engineering, Faculty of Engineering, Anadolu University, Eskisehir, Turkey. · Department of Surgical Oncology, Faculty of Medicine, Ankara University, Ankara, Turkey. ·World J Surg · Pubmed #29750327.

ABSTRACT: PURPOSE: Inguinal hernia repairs are the most common interventions in adults in general surgery clinics. Depending on the type of mesh and repair, the incidence of mesh-related infection ranges from 0.6 to 8%. Methicillin-resistant Staphylococcus aureus is the most common microorganism causing graft infection. The aim of this study was to investigate the efficacy of nano-crystalline silver-coated polypropylene grafts against graft infection created with MRSA in rats. METHODS: A total of 60 female, Wistar albino rats were used in the study. Polypropylene grafts 1 × 1 cm in size were coated in silver ion-doped, calcium phosphate-based, antibacterial ceramic powder (NS-coated graft) to provide an antimicrobial effect. The MRSA seeding procedure was applied at the same time as surgery. In Group 1, normal graft was applied without MRSA seeding, in Group 2, normal graft with MRSA seeding, in Group 3, NS-coated graft without MRSA seeding, and in Group 4, NS-coated graft with MRSA seeding. For the groups which were to be infected, the bacteria were seeded in the surgical area during the operation. On the 7th day postoperatively, all the animals were killed. The grafts were removed and one from each group was examined under electron microscope and the others were implanted in culture medium and the number of colonies was counted after 24 h. RESULTS: In Groups 1 and 3, the incision site was seen to have healed on day 3, no clinical surgical area infection was seen during follow-up, and in the exploration made on the 7th day, no findings of infection were observed. In Group 2, hyperemia and collection were seen to have formed on day 3, abscess had started to form in all the rats of this group on day 4, a purulent discharge in the wound site had started in 12 animals on day 5, separation of the wound site was observed in 6 on day 6, and in the exploration on day 7, there was seen to be a fibrin and pus-rich collection around the graft in all cases. In Group 4, there were hyperemia and collection in 6 animals on day 4, and in 3 of these, abscess was seen to have formed on the 5th day. No purulent discharge or wound separation was observed. In the exploration on the 7th day, it was seen that in the animals with abscess development, the formation was of a localized abscess. The results of the cultures of the grafts removed from Groups 1 and 3 showed no production, whereas production was seen in all the grafts removed from Groups 2 and 4. Clinical surgical area infection was seen to have developed in 100% of Group 2 and in 40% of Group 4. In the comparison of the number of colonies, a statistically significantly lower number of bacteria were determined in Group 4 compared to Group 2 (p < 0.05). In the SEM images taken of Group 2, bacteria clusters were seen attached to the graft. CONCLUSION: Consistent with previous findings in the literature, the NS-coated polypropylene graft was seen to have a significantly better bactericidal effect than the normal polypropylene graft. Development of NS-coated grafts seems to be a reliable and applicable method to reduce the incidence of postoperative graft infection.

2 Article Identification of methicillin-resistant Staphylococcus aureus carrying an exfoliative toxin A gene encoding phage isolated from a hospitalized patient in Turkey. 2013

Sahin, Fikret / Karasartova, Djursun / Özsan, T Murat / Kiyan, Mehmet / Karahan, Ceren Z / Tekeli, Alper. ·Ankara University Faculty of Medicine, Microbiology Department, Ankara, Turkey. fsahin29@hotmail.com ·Can J Microbiol · Pubmed #23586750.

ABSTRACT: From the four known isoforms of the staphylococcal exfoliative toxins (ETs), only ETA and ETB are the major causative agents. General knowledge is that the gene for ETA is located on the chromosome, whereas that for ETB is located on a large plasmid. Yoshizawa and co-workers (2000, Microbiol. Immunol. 44(3): 189-191) isolated, for the first time, a temperate phage (φETA) that carried the structural gene for ETA from an ETA-producing strain of Staphylococcus aureus. In this study, we presented eta gene encoding temperate phages isolated from methicillin-resistant S.aureus (MRSA) isolates obtained from patients in a Turkish hospital. Molecular analysis of the phage genome revealed that the eta gene is located upstream to amidase and holin genes, the same as in the φETA genome. However, partial sequence analysis of amidase and holin genes revealed polymorphic variation. In addition to polymorphic variation, restriction fragment length polymorphism (RFLP) analysis of all of the phage genomes showed that the ETA-containing phage is different from the rest of the phage genomes. The phylogenetic dendrogram of pulsed field gel electrophoresis (PFGE) analysis showed that the ETA-carrying MRSA is quite different from the rest of the MRSA strains. This is the first report showing that a MRSA strain carries an ETA-encoding phage.

3 Article [Identification of a novel lytic bacteriophage obtained from clinical MRSA isolates and evaluation of its antibacterial activity]. 2013

Sahin, Fikret / Karasartova, Djursun / Ozsan, T Murat / Gerçeker, Devran / Kıyan, Mehmet. ·Ankara University Faculty of Medicine, Department of Medical Microbiology, Ankara, Turkey. fsahin29@hotmail.com. ·Mikrobiyol Bul · Pubmed #23390900.

ABSTRACT: Multidrug-resistant bacteria particularly MRSA is well known as a worldwide problem. Since the rate of development of novel antimicrobial agents has been slowed down during the last years, there have been a need for the exploration of alternative solutions for the treatment of resistant bacterial infections. Treatment of infections by bacteriophages (phages) that specifically kill the infecting pathogen, i.e. by the process known as phage therapy, is considered as a possible approach to treat multidrug resistant bacteria. Phage treatment has also been considered to treat Staphylococcus aureus infections. This study was aimed to evaluate the antibacterial and cytotoxic activities of a new lytic phage obtained from clinical MRSA strains. This lytic phage named as f LizAnk was obtained during the phage infectivity studies performed with 13 lysogenic phages against MRSA strains. The antibacterial activity of the f LizAnk phage was determined in vitro in BHI (Brain Heart Infusion) and LB (Leuria Bertani) broths and the in vivo antibacterial activity against MRSA strains and possible cytotoxic effect against mammalian cells were tested on fibroblastic cell cultures (3T3). This study was conducted using 20 MRSA strains isolated from hospitalized patients. Identification of the isolates was performed by conventional methods and methicillin resistance was detected with oxacillin disk diffusion test and mecA gene detection by PCR. The method described by Kaneko et al. [Biosci Biotechnol Biochem 1997; 61(11): 1960-2] was used with some modifications, for induction and isolation of the phages. In vitro studies indicated that this phage killed the six different MRSA strains (in 107 cfu/ml concentrations) in 8 hours, and this powerful lytic effect was similar in both of the liquid media. In vivo studies were performed by using cell cultures prepared in microplates, and the wells have been inoculated with only phage, phage + MRSA mixture, and only MRSA. The cells were then evaluated microscopically as well as by MTT assay which detected alive cells colorimetrically, at 2nd and 24th hours. In our study, the f LizAnk phage did not cause any toxic effect on fibroblast cell cultures, in addition it was observed that the antibacterial effect of the phage against MRSA has proceeded in the cell culture. In conclusion, since the fLizAnk phage described in this study exhibited strong antibacterial activity against MRSA strains and no cytotoxic effect was detected against mammalian cells, it might be safely used alone or in a phage cocktail to treat skin infection caused by MRSA.