Pick Topic
Review Topic
List Experts
Examine Expert
Save Expert
  Site Guide ··   
Obesity: HELP
Articles by Li Chen
Based on 38 articles published since 2009
(Why 38 articles?)
||||

Between 2009 and 2019, Li Chen wrote the following 38 articles about Obesity.
 
+ Citations + Abstracts
Pages: 1 · 2
1 Review Adipose Tissue-Specialized Immunologic Features Might Be the Potential Therapeutic Target of Prospective Medicines for Obesity. 2017

Yao, Fan / Zhang, Ming / Chen, Li. ·Department of Pharmacology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China. · School of Nursing, Jilin University, Changchun 130021, China. ·J Diabetes Res · Pubmed #28466023.

ABSTRACT: Excessive lipid accumulation in adipose tissue is either the source of obesity or the cause and result of chronic local inflammation, and recent studies indicate that the accumulation may induce many other specialized immunologic features with macrophages and epidemic diseases. We analyze the effective stages of immune cells in adipose tissue, including macrophage recruitment, macrophage polarization, and macrophage-like phenotype preadipocyte possession to find optimal sites as drug targets. Subsequently, some main signaling pathways are summarized in this review, including the AMP-activated protein kinase (AMPK) pathway, the JNK signaling pathway, and a novel one, the Notch signaling pathway. We illustrate all these points in order to determine the general pathogenesis of chronic low-grade local inflammation in adipose tissue and the related signaling pathways. In addition, signal-associated prospective compounds, such as berberine, are summarized and discussed with potential targets in pathogenesis. This might provide some possible thoughts and novel therapies for studying chronic inflammatory diseases, such as insulin resistance and type 2 diabetes mellitus.

2 Review Smith-Magenis syndrome and its circadian influence on development, behavior, and obesity - own experience. 2015

Chen, Li / Mullegama, Sureni V / Alaimo, Joseph T / Elsea, Sarah H. ·Department of Cellular and Genetic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China. · Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA. ·Dev Period Med · Pubmed #26384114.

ABSTRACT: Smith-Magenis syndrome (SMS) is a complex genetic disorder characterized by sleep disturbance, multiple developmental anomalies, psychiatric behavior, and obesity. It is caused by a heterozygous 17p11.2 microdeletion containing the retinoic acid-induced 1 (RAI1) gene or mutation within RAI1. Sleep disorder is one of the most penetrant features of SMS. Molecular genetic studies indicate that RAI1 regulates circadian rhythm genes and when haploinsucient, causes a distorted molecular circadian network that may be the cause of the sleep disturbance and the inverted rhythm of melatonin present in most individuals with SMS. RAI1 also regulates genes involved in development, neurobehavior, and lipid metabolism. Sleep debt, daytime melatonin secretion, and environmental stress often contribute to negative behavior in persons with SMS, and food entrained circadian rhythm also influences food intake behavior and humoral signals, which also affect development and neurobehavior. The cross-talk between circadian rhythm, development, metabolism and behaviors affect the multiple phenotypic outcomes in Smith-Magenis syndrome. These findings shed light on possible effective and personalized drug treatments for SMS patients in the future.

3 Clinical Trial Cigarette Smoking Is Associated with a Lower Prevalence of Newly Diagnosed Diabetes Screened by OGTT than Non-Smoking in Chinese Men with Normal Weight. 2016

Hou, Xuhong / Qiu, Jieyuzhen / Chen, Peizhu / Lu, Jun / Ma, Xiaojing / Lu, Juming / Weng, Jianping / Ji, Linong / Shan, Zhongyan / Liu, Jie / Tian, Haoming / Ji, Qiuhe / Zhu, Dalong / Ge, Jiapu / Lin, Lixiang / Chen, Li / Guo, Xiaohui / Zhao, Zhigang / Li, Qiang / Zhou, Zhiguang / Yang, Wenying / Jia, Weiping / Anonymous7280860. ·Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Diabetes Institute, Shanghai Clinical Center for Diabetes, Shanghai, China. · Department of Endocrinology and Metabolism, Chinese People's Liberation Army General Hospital, Beijing, China. · Department of Endocrinology and Metabolism, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. · Department of Endocrinology and Metabolism, Peking University People's Hospital, Beijing, China. · Department of Endocrinology and Metabolism, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China. · Department of Endocrinology and Metabolism, Shanxi Provincial People's Hospital, Taiyuan, Shanxi, China. · Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, Sichuan, China. · Department of Endocrinology and Metabolism, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China. · Department of Endocrinology and Metabolism, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu, China. · Department of Endocrinology and Metabolism, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China. · Department of Endocrinology and Metabolism, Fujian Provincial Hospital, Fuzhou, Fujian, China. · Department of Endocrinology and Metabolism, Qilu Hospital of Shandong University, Jinan, Shandong, China. · Department of Endocrinology and Metabolism, Peking University First Hospital, Beijing, China. · Department of Endocrinology and Metabolism, Henan Provincial People's Hospital, Zhengzhou, Henan, China. · Department of Endocrinology and Metabolism, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. · Department of Endocrinology and Metabolism, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China. · Department of Endocrinology and Metabolism, China-Japan Friendship Hospital, Beijing, China. ·PLoS One · Pubmed #26954355.

ABSTRACT: Different studies have produced conflicting results regarding the association between smoking and diabetes mellitus, and detailed analysis of this issue in Chinese males based on nationwide samples is lacking. We explored the association between cigarette smoking and newly-diagnosed diabetes mellitus (NDM) in Chinese males using a population-based case-control analysis; 16,286 male participants without previously diagnosed diabetes were included. Prediabetes and NDM were diagnosed using the oral glucose tolerance test. The cohort included 6,913 non-smokers (42.4%), 1,479 ex-smokers (9.1%) and 7,894 current smokers (48.5%). Age-adjusted glucose concentrations (mmol/L) were significantly lower at fasting and 120 min in current smokers than non-smokers (5.25 vs. 5.30, 6.46 vs. 6.55, respectively, both P < 0.01). After adjustment for demographic and behavioral variables (age, region, alcohol consumption status, physical activity, education, and family history of diabetes), logistic regression revealed significant negative associations between smoking and NDM in males of a normal weight (BMI < 25 kg/m2: adjusted odds ratio [AOR] = 0.75, P = 0.007; waist circumference < 90 cm: AOR = 0.71, P = 0.001) and males living in southern China (AOR = 0.75, P = 0.009), but not in males who were overweight/obese, males with central obesity, or males living in northern China. Compared to non-smokers, current smokers were less likely to be centrally obese or have elevated BP (AOR: 0.82 and 0.74, both P < 0.05), and heavy smokers (≥ 20 pack-years) were less likely to have elevated TG (AOR = 0.84, P = 0.012) among males of a normal weight. There were no significant associations between quitting smoking and metabolic disorders either among males of a normal weight or males who were overweight/obese. In conclusion, smokers have a lower likelihood of NDM than non-smokers among Chinese males with a lower BMI/smaller waist.

4 Clinical Trial Impact of waist circumference and body mass index on risk of cardiometabolic disorder and cardiovascular disease in Chinese adults: a national diabetes and metabolic disorders survey. 2013

Hou, Xuhong / Lu, Juming / Weng, Jianping / Ji, Linong / Shan, Zhongyan / Liu, Jie / Tian, Haoming / Ji, Qiuhe / Zhu, Dalong / Ge, Jiapu / Lin, Lixiang / Chen, Li / Guo, Xiaohui / Zhao, Zhigang / Li, Qiang / Zhou, Zhiguang / Shan, Guangliang / Yang, Zhaojun / Yang, Wenying / Jia, Weiping / Anonymous4910753. ·Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Diabetes Institute, Shanghai Clinical Center for Diabetes, Shanghai, China. ·PLoS One · Pubmed #23520466.

ABSTRACT: BACKGROUND: We updated the prevalence of obesity and evaluated the clinical utility of separate and combined waist circumference (WC) or body mass index (BMI) category increments in identifying cardiometabolic disorder (CMD) and cardiovascular disease (CVD) risk in Chinese adults. METHODS AND FINDINGS: 46,024 participants aged ≥20 years, a nationally representative sample surveyed in 2007-2008, were included in this analysis. Taking the cutoffs recommended by the Chinese Joint Committee for Developing Chinese Guidelines (JCDCG) and the Working Group on Obesity in China (WGOC) into account, the participants were divided into four WC and four BMI groups in 0.5-SD increments around the mean, and 16 cross-tabulated combination groups of WC and BMI. 27.1%, 31.4%, and 12.2% of Chinese adults are centrally obese, overweight, or obese according to JCDCG and WGOC criteria. After adjustment for confounders, after a 1-SD increment, WC is associated with a 1.7-fold or 2.2-fold greater risk of having DM or DM plus dyslipidemia than BMI, while BMI was associated with a 2.3-fold or 1.7-fold higher hypertension or hypertension plus dyslipidemia risk than WC. The combination of WC and BMI categories had stronger association with CMD risk, i.e., the adjusted ORs (95% CI) of having DM, hypertension, and dyslipidemia for the combined and separate highest WC and BMI categories were 2.19 (1.96-2.44) vs 1.88 (1.67-2.12) and 1.12 (0.99-1.26); 5.70 (5.24-6.19) vs 1.51 (1.39-1.65) and 1.69 (1.57-1.82); and 3.73 (3.42-4.07) vs 2.16 (1.98-2.35) and 1.33 (1.25-1.40), respectively. The combination of WC and BMI categories was more likely to identify individuals with lower WC and lower BMI at CVD risk, even after the effects of CMD were controlled (all P<0.05). CONCLUSION: Central obesity, overweight, and obesity are epidemic in Chinese adults. The combination of WC and BMI measures is superior to the separate indices in identifying CMD and CVD risk.

5 Article [Electroacupuncture reduces obesity by improving metabolism and up-regulating expression of hypothalamic Sirtuin 1 and proopiomelanocortin in obese rats]. 2019

Huang, Qi / Chen, Rui / Chen, Li / Liang, Feng-Xia / He, Wen-Juan / Peng, Miao / Li, Lun. ·College of Acupuncture-moxibustion and Orthopaedics of Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-moxibustion, Wuhan 430061, China. · Department of Integrated Traditional Chinese and Western Medicine, the Union Hospital Affiliated to Tongji Medical College of Huazhong Science and Technology University, Wuhan 430022. · Department of Rehabilitation, the Ninth Hospital of Wuhan, Wuhan 430081. ·Zhen Ci Yan Jiu · Pubmed #31056880.

ABSTRACT: OBJECTIVE: To observe the effect of electroacupuncture (EA) on expression of hypothalamic sirtuin 1(SIRT1) and proopiomelanocortin (POMC), and body weight, food-intake, blood glucose, and blood lipid levels in obese rats, so as to explore its mechanisms underlying improvement of obesity. METHODS: Forty male Wistar rats were randomly divided into normal, model, EA, and sham EA groups ( RESULTS: In comparison with the normal group, the body weight, food-intake, blood lipids, and PPG levels were significantly increased ( CONCLUSION: EA can reduce the obese rats' body weight, food-intake, blood lipids and blood glucose, which may be associated with its effect in up-regulating the SIRT1 and POMC expression of hypothalamus.

6 Article Fluvastatin Sodium Ameliorates Obesity through Brown Fat Activation. 2019

Yin, Na / Zhang, Hanlin / Ye, Rongcai / Dong, Meng / Lin, Jun / Zhou, Huiqiao / Huang, Yuanyuan / Chen, Li / Jiang, Xiaoxiao / Nagaoka, Kentaro / Zhang, Chuanhai / Jin, Wanzhu. ·Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. yinna@ioz.ac.cn. · University of the Chinese Academy of Sciences, Beijing 100049, China. yinna@ioz.ac.cn. · Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. zhanghanlin@ioz.ac.cn. · University of the Chinese Academy of Sciences, Beijing 100049, China. zhanghanlin@ioz.ac.cn. · Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. yerongcai@126.com. · University of the Chinese Academy of Sciences, Beijing 100049, China. yerongcai@126.com. · Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. dongmeng90@foxmail.com. · University of the Chinese Academy of Sciences, Beijing 100049, China. dongmeng90@foxmail.com. · Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. cleverlinjun@126.com. · University of the Chinese Academy of Sciences, Beijing 100049, China. cleverlinjun@126.com. · Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. jessicachou36@126.com. · University of the Chinese Academy of Sciences, Beijing 100049, China. jessicachou36@126.com. · Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. lvhaizi@sina.com. · University of the Chinese Academy of Sciences, Beijing 100049, China. lvhaizi@sina.com. · Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. 18811322312@163.com. · University of the Chinese Academy of Sciences, Beijing 100049, China. 18811322312@163.com. · Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. xiaoxiao0826@126.com. · University of the Chinese Academy of Sciences, Beijing 100049, China. xiaoxiao0826@126.com. · Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan. nagaokak@cc.tuat.ac.jp. · Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. zhangchuanhai.68@163.com. · Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. jinw@ioz.ac.cn. · University of the Chinese Academy of Sciences, Beijing 100049, China. jinw@ioz.ac.cn. ·Int J Mol Sci · Pubmed #30939798.

ABSTRACT: Brown adipose tissue (BAT), an organ that burns energy through uncoupling thermogenesis, is a promising therapeutic target for obesity. However, there are still no safe anti-obesity drugs that target BAT in the market. In the current study, we performed large scale screening of 636 compounds which were approved by Food and Drug Administration (FDA) to find drugs that could significantly increase uncoupling protein 1 (

7 Article Self-reported snoring is associated with chronic kidney disease independent of metabolic syndrome in middle-aged and elderly Chinese. 2019

Song, Jun / Wang, Chuan / Ma, Aixia / Zheng, Huizhen / Zheng, Wenjian / Hou, Xinguo / Hu, Cheng / Chen, Li / Jia, Weiping. ·Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China. · Department of Endocrinology, Qilu Hospital of Shandong University, Jinan, Shandong, China. · Department of Geriatrics, Qingdao Haici Medical Treatment Group, Qingdao, Shandong, China. ·J Diabetes Investig · Pubmed #29694704.

ABSTRACT: AIMS/INTRODUCTION: To investigate the correlation between snoring and chronic kidney disease (CKD), and explore whether metabolic syndrome (MetS) plays an important role in this relationship among middle-aged and elderly Chinese. MATERIALS AND METHODS: The participants included in the present study were categorized into three subgroups based on self-reported snoring frequency (regularly [≥3 times per week], occasionally [between 'regularly' and 'never'] or never [<1 time per month]). An estimated glomerular filtration rate <60 mL/min/1.73 m RESULTS: The frequency of MetS, MetS components and CKD was dramatically higher in regular snorers than in non-snorers and occasional snorers. The odds ratios for MetS and all the MetS elements, except for hyperglycemia, increased progressively with the snoring frequency (P < 0.001). Upon additional adjustment for other MetS components, snoring was not significantly related with hypertension; however, the associations between snoring frequency and overweight/obesity and dyslipidemia became attenuated, but still remained statistically significant (P < 0.01). Interestingly, odds ratios for CKD also increasingly augmented with snoring frequency (P < 0.001). Upon further adjustment for individual MetS components or MetS, regular snoring also resulted in a significantly increased odds ratio for CKD (odds ratio 1.72; P = 0.034) relative to non-snoring. CONCLUSIONS: Self-reported snoring is closely associated with CKD independent of MetS among middle-aged and elderly Chinese.

8 Article Impact of sex, body mass index and initial pathologic diagnosis age on the incidence and prognosis of different types of cancer. 2018

Huang, Xuan / Shu, Chuanjun / Chen, Li / Yao, Bing. ·Reproductive Medical Center, Jinling Hospital Affiliated to The Medical School of Nanjing University, Nanjing, Jiangsu 210002, P.R. China. · State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu 210096, P.R. China. · Reproductive Medical Center, Jinling Hospital Affiliated to The Medical School of Nanjing University, Nanjing, Jiangsu 210002, P.R. China. ·Oncol Rep · Pubmed #29956810.

ABSTRACT: Cancer represents a significant challenge for humankind, as early diagnosis and treatment are difficult to achieve. To systemically investigate the effect of sex, body mass index (BMI) and age on cancer incidence and prognosis, the data from 14,504 cases of cancer were downloaded from The Cancer Genome Atlas (TCGA). BMI was used to categorize each person as underweight, normal weight, overweight or obese. Two‑ and five‑year survival rates were applied to estimate the prognosis for each cancer type. All data were statistically analyzed. We identified that males were more susceptible to lung, liver and skin cancer when compared with females, whereas females were more susceptible to thyroid, breast and adrenal cortex cancer. High BMI (>25) was positively associated with the occurrence of cancer, although patients with high BMI at the time of initial diagnosis had higher two/five‑year survival rates. The survival rates for cancer were positively correlated with the age at initial pathologic diagnosis. Some types of cancer were associated with particularly young ages of onset, including adrenocortical carcinoma, cervical and endocervical cancers, brain lower grade glioma, pheochromocytoma and paraganglioma, testicular germ cell tumors and thyroid carcinoma. Hence, the early diagnosis and prognosis for these cancers need to be improved. In conclusion, sex, BMI and age are associated with the incidence and survival rates for cancers. These results could be used to supplement precision and personalized medicine.

9 Article Adipocyte-Derived Exosomal MiR-27a Induces Insulin Resistance in Skeletal Muscle Through Repression of PPARγ. 2018

Yu, Yang / Du, Hongwei / Wei, Shengnan / Feng, Linjing / Li, Junnan / Yao, Fan / Zhang, Ming / Hatch, Grant M / Chen, Li. ·Department of Pharmacology, College of Basic Medical Sciences, School of nursing, Jilin University, Changchun 130021, China. · Research Institution of Paediatrics, Department of Pediatric Endocrinology, The First Clinical Hospital Affiliated to Jilin University, Changchun, Jilin 130021, China. · DREAM, Children's Hospital Research Institute of Manitoba, Center for Research and Treatment of Atherosclerosis, Department of Pharmacology & Therapeutics, University of Manitoba, Winnipeg, Manitoba, Canada. ·Theranostics · Pubmed #29721071.

ABSTRACT: The mechanism by which adipocyte-derived endocrine factors promote insulin resistance in skeletal muscle are not fully understood. MiR-27a is highly expressed in sera of obese individuals with prediabetes and T2DM, and mainly derived by adipose tissues. Thus, miR-27a secreted into circulation by adipose tissue may regulate insulin resistance in skeletal muscle.

10 Article Analysis of human sperm DNA fragmentation index (DFI) related factors: a report of 1010 subfertile men in China. 2018

Lu, Jin-Chun / Jing, Jun / Chen, Li / Ge, Yi-Feng / Feng, Rui-Xiang / Liang, Yuan-Jiao / Yao, Bing. ·The Reproductive Medical Centre, Nanjing Jinling Hospital, Nanjing University School of Medicine, 305 Zhongshan East Road, Nanjing, 210002, Jiangsu, China. · Department of Laboratory Science, Nanjing Hospital, Jiangsu Corps, The Armed Police Force, PLA, Nanjing, 210028, Jiangsu, China. · The Reproductive Medical Centre, Nanjing Jinling Hospital, Nanjing University School of Medicine, 305 Zhongshan East Road, Nanjing, 210002, Jiangsu, China. 2424572228@qq.com. ·Reprod Biol Endocrinol · Pubmed #29540184.

ABSTRACT: BACKGROUND: Many factors may lead to sperm DNA damage. However, it is little known that the correlations of sperm DNA damage with obesity-associated markers, and reproductive hormones and lipids levels in serum and seminal plasma. METHODS: In our prospective study, a total of 1 010 subfertile men, aged from 18 to 50 years old, were enrolled from August 2012 through June 2015. Their obesity-associated markers, semen parameters, sperm acrosomal enzyme activity, seminal plasma biochemical markers, and reproductive hormones and lipids levels in serum and seminal plasma were detected. Sperm DNA fragmentation index (DFI) was determined by sperm chromatin structure assay. The correlations between DFI and each of the above-mentioned variables were analyzed. RESULTS: Spearman correlation analysis showed that sperm DFI was positively related to age and abstinence time (P<0.001). Sperm DFI was also positively related to semen volume and percent of abnormal sperm head (P<0.001), while negatively related to sperm concentration, progressive motility (PR), sperm motility, total normal-progressively motile sperm count (TNPMS), percent of normal sperm morphology (NSM), percent of intact acrosome and acrosomal enzyme activity (P<0.001). Sperm DFI was positively related to seminal plasma zinc level (P<0.001) but unrelated to seminal plasma total α-glucotase, γ-glutamyl transpeptidase (GGT) and fructose levels. There was no any correlation between sperm DFI and obesity-associated markers such as body mass index (BMI), waist-to-hip ratio (WHR), waist circumference (WC) and waist-to-height ratio (WHtR), and serum lipids levels, but there was positive correlation between sperm DFI and seminal plasma triglyceride (TG) and total cholesterol (TC) levels (P<0.001). Sperm DFI was positively related to serum luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels and seminal plasma FSH and estradiol (E2) levels (P<0.001), but unrelated to serum and seminal plasma testosterone (T) levels. The multivariate regression analysis for the variables which were significantly correlated with sperm DFI in Spearman correlation analysis showed that age, semen volume, sperm concentration, progressive motility, TNPMS and intact acrosome were independently correlated with sperm DFI. CONCLUSIONS: There are many potential factors associated with sperm DFI, including age, abstinence time, spermatogenesis and maturation, seminal plasma lipids and reproductive hormones levels. However, the potential effects of seminal plasma lipids and reproductive hormones on sperm DNA damage need still to be demonstrated by the studies with scientific design and a large size of samples.

11 Article PINK1-Parkin alleviates metabolic stress induced by obesity in adipose tissue and in 3T3-L1 preadipocytes. 2018

Cui, Chen / Chen, Shihong / Qiao, Jingting / Qing, Li / Wang, Lingshu / He, Tianyi / Wang, Chuan / Liu, Fuqiang / Gong, Lei / Chen, Li / Hou, Xinguo. ·Department of Endocrinology, Qilu Hospital of Shandong University, Jinan 250012, China. · Department of Endocrinology, The Second Hospital of Shandong University, Jinan, 250033, China. · Department of Endocrinology, Qilu Hospital of Shandong University, Jinan 250012, China; Institute of Endocrine and Metabolic Disease, Shandong University, Jinan 250012, China; Key Laboratory of Endocrinology and Metabolism, Shandong Province in Medicine &Health, Jinan 250012, China. · Department of Endocrinology, Qilu Hospital of Shandong University, Jinan 250012, China; Institute of Endocrine and Metabolic Disease, Shandong University, Jinan 250012, China; Key Laboratory of Endocrinology and Metabolism, Shandong Province in Medicine &Health, Jinan 250012, China. Electronic address: houxinguo@sdu.edu.cn. ·Biochem Biophys Res Commun · Pubmed #29501495.

ABSTRACT: Mitochondria play an important role in cellular metabolism and are closely related with metabolic stress. Recently, several studies have shown that mitophagy mediated by PTEN-induced putative kinase 1 (PINK1) and Parkin may play a critical role in clearing the damaged mitochondria and maintaining the overall balance of intracellular mitochondria in quality and quantity. A previous study showed that PINK1 and Parkin were overexpressed in adipose tissue in obese subjects. However, it is still unclear whether a direct relationship exists between obesity and mitophagy. In this study, we created a high-fat-diet (HFD)-induced obese mouse model and examined the expression of PINK1 and Parkin in adipose tissue using western blot and real-time quantitative PCR. After we confirmed that there is an interesting difference between regular-chow-fed mice and HFD-induced obese mice in the expression of PINK1 and Parkin in vivo, we further tested the expression of PINK1 and Parkin in 3T3-L1 preadipocytes in vitro by treating cells with palmitic acid (PA) to induce metabolic stress. To better understand the role of PINK1 and Parkin in metabolic stress, 3T3-L1 preadipocytes were transfected with small interfering RNA (siRNA) of PINK1 and Parkin followed by PA treatment. Our results showed that under lower concentrations of PA, PINK1 and Parkin can be activated and play a protective role in resisting the harmful effects of PA, including protecting the mitochondrial function and resisting cellular death, while under higher concentrations of PA, the expression of PINK1 and Parkin can be inhibited. These results suggest that PINK1-Parkin can protect mitochondrial function against metabolic stress induced by obesity or PA to a certain degree.

12 Article Cholesterol metabolism and Cx43, Cx46, and Cx50 gap junction protein expression and localization in normal and diabetic and obese ob/ob and db/db mouse testes. 2018

Pelletier, R-Marc / Akpovi, Casimir D / Chen, Li / Vitale, María Leiza. ·Department of Pathology and Cell Biology, Université de Montréal , Montréal, Québec , Canada. ·Am J Physiol Endocrinol Metab · Pubmed #28851737.

ABSTRACT: Decreased fertility and birth rates arise from metabolic disorders. This study assesses cholesterol metabolism and Cx46, Cx50, and Cx43 expression in interstitium- and seminiferous tubule-enriched fractions of leptin-deficient ( ob/ob) and leptin receptor-deficient ( db/db) mice, two type 2 diabetes and obesity models associated with infertility. Testosterone levels decreased and glucose and free and esterified cholesterol (FC and EC) levels increased in serum, whereas FC and EC levels decreased in the interstitium, in ob/ob and db/db mice. In tubules, a decrease in EC caused FC-to-EC ratios to increase in db/db mice. In tubules, only acyl coenzyme A:cholesterol acyl transferase type 1 and 2 protein levels significantly decreased in ob/ob, but not db/db, mice compared with wild-type mice, and imbalances in the cholesterol transporters Niemann-Pick C1 (NPC1), ATP-binding cassette A1 (ABCA1), scavenger receptor class B member I (SR-BI), and cluster of differentiation 36 (CD36) were observed in ob/ob and db/db mice. In tubules, 14-kDa Cx46 prevailed during development, 48- to 49- and 68- to 71-kDa Cx46 prevailed during adulthood, and total Cx46 changed little. Compared with wild-type mice, 14-kDa Cx46 increased, whereas 48- to 49- and 68- to 71-kDa Cx46 decreased, in tubules, whereas the opposite occurred in the interstitium, in db/db and ob/ob mice. Total and 51-kDa Cx50 increased in db/db and ob/ob interstitium and tubules. Cx43 levels decreased in ob/ob interstitium and tubules, whereas Cx43 decreased in db/db interstitium but increased in db/db tubules. Apoptosis levels measured by ELISA and numbers of apostain-labeled apoptotic cells significantly increased in db/db, but not ob/ob, tubules. Testicular db/db capillaries were Cx50-positive but weakly Cx43-positive with a thickened lamina, suggesting altered permeability. Our findings indicate that the db mutation-induced impairment of meiosis may arise from imbalances in cholesterol metabolism and upregulated Cx43 expression and phosphorylation in tubules.

13 Article Adipogenic miR-27a in adipose tissue upregulates macrophage activation via inhibiting PPARγ of insulin resistance induced by high-fat diet-associated obesity. 2017

Yao, Fan / Yu, Yang / Feng, Linjing / Li, Junnan / Zhang, Meishuang / Lan, Xiaoxin / Yan, Xin / Liu, Yilun / Guan, Fengying / Zhang, Ming / Chen, Li. ·Department of Pharmacology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China. · Department of Pharmacology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China. Electronic address: zhangming_00@126.com. · Department of Pharmacology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China; School of Nursing, Jilin University, Changchun 130021, China. Electronic address: chen_lab@163.com. ·Exp Cell Res · Pubmed #28365247.

ABSTRACT: Chronic low degree inflammation caused by macrophage activation is a crucial factor underlying insulin resistance induced by obesity. To illustrate the mechanism of regulating of macrophage activation in adipose tissue, the role of adipogenic miR-27a activating M1 macrophage polarization via blocking PPARγ was evaluated. Obese mice model and miR-27a overexpression or knockdown mice model were established and related biochemical index were examined. Raw264.7 and 3T3-L1 were cultured and co-cultured for mimicking the microenvironment of local inflammation. Macrophage infiltration was observed. MiR-27a and cytokines levels in serum and adipose tissue were measured. Macrophage polarization markers and protein expression in insulin or inflammatory signaling pathways were observed. Impaired glucose tolerance and insulin tolerance was observed in 4w, 8w and 12w of high fat diet and miR-27a overexpression mice. Concurrently, miR-27a was increased in serum in a time-dependent manner, along with M1 cytokines and M1 macrophages increasing in adipose tissue clearly. Insulin signaling pathway was blocked, and PPARγ was suppressed. However, NF-κB was activated. On the other hand, activated macrophages and hypertrophic adipocytes induced by miR-27a could increase the ratio of Raw264.7 migration, including improving cytokines generation, and blocking PPARγ expression markedly. The present studies are conducted to clarify that miR-27a has increased along with up-regulation in the process of proinflammatory cytokines generation, macrophage influx and M1 macrophage polarization in obesity. These indicate that miR-27a gives the novel target of intervention for inflammation and insulin resistance in obesity.

14 Article Effect of Roux-en-Y gastric bypass on carotid intima-media thickness in Chinese obese patients with type 2 diabetes. 2017

Chen, Jie / Yu, Haoyong / Chen, Li / Wu, Lei / Hu, Bing / Bao, Yuqian / Jiang, Lixin. ·Department of Ultrasound in Medicine, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai Institute of Ultrasound in Medicine, Shanghai, China. · Department of Endocrinology and Metabolism, Shanghai Diabetes Institute, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China. · Department of Endocrinology and Metabolism, Shanghai Diabetes Institute, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China. Electronic address: byq522@163.com. · Department of Ultrasound in Medicine, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai Institute of Ultrasound in Medicine, Shanghai, China. Electronic address: chen1978910@163.com. ·Surg Obes Relat Dis · Pubmed #28363402.

ABSTRACT: BACKGROUND: The effect of Roux-en-Y gastric bypass (RYGB) on carotid intima-media thickness (C-IMT) in obese patients with type 2 diabetes (T2D) has not been well studied. OBJECTIVE: To investigate the effect of RYGB on C-IMT and the relationship between C-IMT and some cardiovascular risk factors 12 months after surgery on Chinese obese patients with T2D. SETTING: University hospital, China. METHODS: Thirty-three consecutive obese patients with T2D and 18 healthy volunteers matched in age and sex were enrolled in this retrospective study. The anthropometric data, metabolism variables, and C-IMT were recorded before and 12 months after RYGB. RESULTS: The patient group had significantly higher C-IMT than the control group before surgery (589.5±96.9 μm versus 457.9±48.6 μm, respectively; P<.01). C-IMT had significant positive relationships with age, body mass index, systolic blood pressure, visceral fat area (VFA), and serum leptin (r = .56, .44, .38, .42, and .38, respectively; P<.01). Twelve months after RYGB, the anthropometric data, metabolism variables, and C-IMT had improved significantly. The change in C-IMT was positively correlated with changes in VFA and leptin level (r = .39 and .36, respectively; P<.01). CONCLUSION: RYGB surgery seems to be effective on C-IMT for obese patients with T2D in China. VFA and leptin level are related to improvement of C-IMT.

15 Article Developmental pathways to adiposity begin before birth and are influenced by genotype, prenatal environment and epigenome. 2017

Lin, Xinyi / Lim, Ives Yubin / Wu, Yonghui / Teh, Ai Ling / Chen, Li / Aris, Izzuddin M / Soh, Shu E / Tint, Mya Thway / MacIsaac, Julia L / Morin, Alexander M / Yap, Fabian / Tan, Kok Hian / Saw, Seang Mei / Kobor, Michael S / Meaney, Michael J / Godfrey, Keith M / Chong, Yap Seng / Holbrook, Joanna D / Lee, Yung Seng / Gluckman, Peter D / Karnani, Neerja / Anonymous6540898. ·Singapore Institute for Clinical Sciences, A*STAR, 30 Medical Drive, Singapore, 117609, Singapore. · Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 119228, Singapore. · Department of Pediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 119228, Singapore. · Centre for Molecular Medicine and Therapeutics, Child and Family Research Institute, Department of Medical Genetics, University of British Columbia, Vancouver, BC, V5Z 4H4, Canada. · KK Women's and Children's Hospital, Singapore, 229899, Singapore. · Saw Swee Hock School of Public Health, National University of Singapore, Singapore, 117597, Singapore. · Singapore Eye Research Institute, Singapore, 169856, Singapore. · Duke NUS Medical School, Singapore, 169857, Singapore. · Ludmer Centre for Neuroinformatics and Mental Health, Douglas University Mental Health Institute, McGill University, Montreal, Quebec, H4H 1R3, Canada. · MRC Lifecourse Epidemiology Unit and NIHR Southampton Biomedical Research Centre, University of Southampton and University Hospital Southampton NHS Foundation Trust, Southampton, SO16 6YD, UK. · Division of Paediatric Endocrinology and Diabetes, Khoo Teck Puat-National University Children's Medical Institute, National University Health System, Singapore, 119228, Singapore. · Centre for Human Evolution, Adaptation and Disease, Liggins Institute, University of Auckland, Auckland, 1142, New Zealand. · Singapore Institute for Clinical Sciences, A*STAR, 30 Medical Drive, Singapore, 117609, Singapore. neerja_karnani@sics.a-star.edu.sg. · Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 119228, Singapore. neerja_karnani@sics.a-star.edu.sg. ·BMC Med · Pubmed #28264723.

ABSTRACT: BACKGROUND: Obesity is an escalating health problem worldwide, and hence the causes underlying its development are of primary importance to public health. There is growing evidence that suboptimal intrauterine environment can perturb the metabolic programing of the growing fetus, thereby increasing the risk of developing obesity in later life. However, the link between early exposures in the womb, genetic susceptibility, and perturbed epigenome on metabolic health is not well understood. In this study, we shed more light on this aspect by performing a comprehensive analysis on the effects of variation in prenatal environment, neonatal methylome, and genotype on birth weight and adiposity in early childhood. METHODS: In a prospective mother-offspring cohort (N = 987), we interrogated the effects of 30 variables that influence the prenatal environment, umbilical cord DNA methylation, and genotype on offspring weight and adiposity, over the period from birth to 48 months. This is an interim analysis on an ongoing cohort study. RESULTS: Eleven of 30 prenatal environments, including maternal adiposity, smoking, blood glucose and plasma unsaturated fatty acid levels, were associated with birth weight. Polygenic risk scores derived from genetic association studies on adult adiposity were also associated with birth weight and child adiposity, indicating an overlap between the genetic pathways influencing metabolic health in early and later life. Neonatal methylation markers from seven gene loci (ANK3, CDKN2B, CACNA1G, IGDCC4, P4HA3, ZNF423 and MIRLET7BHG) were significantly associated with birth weight, with a subset of these in genes previously implicated in metabolic pathways in humans and in animal models. Methylation levels at three of seven birth weight-linked loci showed significant association with prenatal environment, but none were affected by polygenic risk score. Six of these birth weight-linked loci continued to show a longitudinal association with offspring size and/or adiposity in early childhood. CONCLUSIONS: This study provides further evidence that developmental pathways to adiposity begin before birth and are influenced by environmental, genetic and epigenetic factors. These pathways can have a lasting effect on offspring size, adiposity and future metabolic outcomes, and offer new opportunities for risk stratification and prevention of obesity. CLINICAL TRIAL REGISTRATION: This birth cohort is a prospective observational study, designed to study the developmental origins of health and disease, and was retrospectively registered on 1 July 2010 under the identifier NCT01174875 .

16 Article Reduced Kidney Function Is Associated With Cardiometabolic Risk Factors, Prevalent and Predicted Risk of Cardiovascular Disease in Chinese Adults: Results From the REACTION Study. 2016

Lu, Jieli / Mu, Yiming / Su, Qing / Shi, Lixin / Liu, Chao / Zhao, Jiajun / Chen, Lulu / Li, Qiang / Yang, Tao / Yan, Li / Wan, Qin / Wu, Shengli / Liu, Yan / Wang, Guixia / Luo, Zuojie / Tang, Xulei / Chen, Gang / Huo, Yanan / Gao, Zhengnan / Ye, Zhen / Wang, Youmin / Qin, Guijun / Deng, Huacong / Yu, Xuefeng / Shen, Feixia / Chen, Li / Zhao, Liebin / Sun, Jichao / Sun, Wanwan / Wang, Tiange / Du, Rui / Lin, Lin / Dai, Meng / Xu, Yu / Xu, Min / Bi, Yufang / Lai, Shenghan / Li, Donghui / Wang, Weiqing / Ning, Guang / Anonymous10870875. ·State Key Laboratory of Medical Genomics, Key Laboratory for Endocrine and Metabolic Diseases of Ministry of Health, National Clinical Research Center for Metabolic Diseases, Collaborative Innovation Center of Systems Biomedicine, Shanghai Jiao-Tong University School of Medicine, Shanghai, China Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai, China. · Chinese People's Liberation Army General Hospital, Beijing, China. · Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China. · Affiliated Hospital of Guiyang Medical College, Guiyang, China. · Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing, China. · Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China. · Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. · The Second Affiliated Hospital of Harbin Medical University, Harbin, China. · The First Affiliated Hospital with Nanjing Medical University, Jiangsu Province Hospital, Nanjing, China. · Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China. · The Affiliated Hospital of Luzhou Medical College, Luzhou, China. · Karamay Municipal People's Hospital, Xinjiang, China. · The First Hospital of Jilin University, Changchun, China. · The First Affiliated Hospital of Guangxi Medical University, Nanning, China. · The First Hospital of Lanzhou University, Lanzhou, China. · Fujian Provincial Hospital, Fujian Medical University, Fuzhou, China. · Jiangxi People's Hospital, Nanchang, China. · Dalian Municipal Central Hospital, Dalian, China. · Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China. · The First Affiliated Hospital of Anhui Medical University, Hefei, China. · The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. · The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. · Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. · The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. · Qilu Hospital of Shandong University, Jinan, China. · Johns Hopkins University School of Medicine, Baltimore, MD. · Department of Gastrointestinal Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. · State Key Laboratory of Medical Genomics, Key Laboratory for Endocrine and Metabolic Diseases of Ministry of Health, National Clinical Research Center for Metabolic Diseases, Collaborative Innovation Center of Systems Biomedicine, Shanghai Jiao-Tong University School of Medicine, Shanghai, China Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai, China wqingw61@163.com. ·J Am Heart Assoc · Pubmed #27451464.

ABSTRACT: BACKGROUND: Chronic kidney disease (CKD) increases cardiovascular disease (CVD) risk. However, the association of mildly reduced kidney function with CVD risk is unclear. METHODS AND RESULTS: This study investigated the association of estimated glomerular filtration rate (eGFR) with prevalent CVDs, 10-year Framingham risk for coronary heart disease (CHD), and 10-year risk of atherosclerotic cardiovascular diseases (ASCVD) in 239 832 participants from the baseline of the Risk Evaluation of cAncers in Chinese diabeTic Individuals: a lONgitudinal study. With an interviewer-assisted questionnaire, we collected information on CVD, including reported CHD, stroke, or myocardial infarction. Chronic Kidney Disease-Epidemiology Collaboration (CKD-EPI) equation was used to calculate eGFR. Compared with individuals with normal eGFR (≥90 mL/min per 1.73 m(2)), those with decreased eGFR (75-89, 60-74, and <60 mL/min per 1.73 m(2)) had higher risk of prevalent obesity, diabetes mellitus, hypertension, and dyslipidemia in both men and women (P for trend all <0.001). Moreover, a significantly higher 10-year Framingham risk for CHD and 10-year risk for ASCVD was observed in both men and women with mildly decreased eGFR (60-89 mL/min per 1.73 m(2)). CONCLUSIONS: Even mildly reduced eGFR (under 90 mL/min per 1.73 m(2)) is associated with elevated 10-year Framingham risk for CHD and 10-year ASCVD risk among Chinese adults.

17 Article SIRT3 Deficiency Induces Endothelial Insulin Resistance and Blunts Endothelial-Dependent Vasorelaxation in Mice and Human with Obesity. 2016

Yang, Lu / Zhang, Julei / Xing, Wenjuan / Zhang, Xing / Xu, Jie / Zhang, Haifeng / Chen, Li / Ning, Xiaona / Ji, Gang / Li, Jia / Zhao, Qingchuan / Gao, Feng. ·Department of Aerospace Medicine, Fourth Military Medical University, 169 Changlexi Road, Xi'an 710032, China. · Department of Physiology, Fourth Military Medical University, 169 Changlexi Road, Xi'an 710032, China. · Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 169 Changlexi Road, Xi'an 710032, China. · Department of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, 169 Changlexi Road, Xi'an 710032, China. ·Sci Rep · Pubmed #27000941.

ABSTRACT: Recent evidence implicates the critical role of Sirtuin 3 (SIRT3) in the development of many metabolic diseases, but the contribution of SIRT3 to vascular homeostasis remains largely unknown. The aim of this study was to investigate the role of SIRT3 in endothelial insulin resistance and vascular dysfunction in obesity. We found an impaired insulin-induced mesenteric vasorelaxation and concomitant reduced vascular SIRT3 expression in morbid obese human subjects compared with the non-obese subjects. Downregulation of SIRT3 in cultured human endothelial cells increased mitochondrial reactive oxygen species (mtROS) and impaired insulin signaling as evidenced by decreased phosphorylation of Akt and endothelial nitric oxide synthase and subsequent reduced nitric oxide (NO) release. In addition, obese mice induced by 24-week high-fat diet (HFD) displayed an impaired endothelium-dependent vasorelaxation to both insulin and acetylcholine, which was further exacerbated by the gene deletion of Sirt3. Scavenging of mtROS not only restored insulin-stimulated NO production in SIRT3 knockdown cells, but also improved insulin-induced vasorelaxation in SIRT3 knockout mice fed with HFD. Taken together, our findings suggest that SIRT3 positively regulates endothelial insulin sensitivity and show that SIRT3 deficiency and resultant increased mtROS contribute to vascular dysfunction in obesity.

18 Article Association between the change in body mass index from early adulthood to midlife and subsequent type 2 diabetes mellitus. 2016

Sun, Wanwan / Shi, Lixin / Ye, Zhen / Mu, Yiming / Liu, Chao / Zhao, Jiajun / Chen, Lulu / Li, Qiang / Yang, Tao / Yan, Li / Wan, Qin / Wu, Shengli / Liu, Yan / Wang, Guixia / Luo, Zuojie / Tang, Xulei / Chen, Gang / Huo, Yanan / Gao, Zhengnan / Su, Qing / Wang, Youmin / Qin, Guijun / Deng, Huacong / Yu, Xuefeng / Shen, Feixia / Chen, Li / Zhao, Liebin / Sun, Jichao / Ding, Lin / Xu, Yu / Xu, Min / Dai, Meng / Wang, Tiange / Zhang, Di / Lu, Jieli / Bi, Yufang / Lai, Shenghan / Li, Donghui / Wang, Weiqing / Ning, Guang / Anonymous190857. ·National Clinical Research Center for Metabolic Diseases, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. · Affiliated Hospital of Guiyang Medical College, Guiyang, China. · Zhejiang Provincial Center for Disease Control and Prevention, China. · Chinese People's Liberation Army General Hospital, Beijing, China. · Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing, China. · Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China. · Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. · The Second Affiliated Hospital of Harbin Medical University, Harbin, China. · The First Affiliated Hospital with Nanjing Medical University, Jiangsu Province Hospital, Nanjing, China. · Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China. · The Affiliated Hospital of Luzhou Medical College, Luzhou, China. · Karamay Municipal People's Hospital, Xinjiang, China. · The First Hospital of Jilin University, Changchun, China. · The First Affiliated Hospital of Guangxi Medical University, Nanning, China. · The First Hospital of Lanzhou University, Lanzhou, China. · Fujian Provincial Hospital, Fujian Medical University, Fuzhou, China. · Jiangxi People's Hospital, Nanchang, China. · Dalian Municipal Central Hospital, Dalian, China. · Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China. · The First Affiliated Hospital of Anhui Medical University, Hefei, China. · The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. · The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. · Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. · The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. · Qilu Hospital of Shandong University, Jinan, China. · Laboratory of Endocrine and Metabolic Diseases, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, and Shanghai Jiao Tong University School of Medicine, Shanghai, China. · Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. · Department of Gastrointestinal Medical Oncology, the University of Texas MD Anderson Cancer Center, Houston, Texas, USA. ·Obesity (Silver Spring) · Pubmed #26833544.

ABSTRACT: OBJECTIVE: To clarify the quantitative relationship of body mass index (BMI) change from early adulthood to midlife with presence of type 2 diabetes mellitus (T2DM) after midlife. METHODS: This study included 120,666 middle-aged and elderly, whose retrospectively self-reported body weight at 20 and 40 years and measured height were available. BMI at 20 and 40 years and BMI change in between were defined as early-adulthood BMI, midlife BMI, and early-adulthood BMI change. RESULTS: The odds ratio (OR) for T2DM associated with an 1-unit increment of early-adulthood or midlife BMI was 1.08 (95% confidence interval (CI), 1.07-1.08) and 1.09 (95% CI, 1.09-1.10) respectively. In the cross-tabulation of both early-adulthood BMI and BMI change, the prevalence of T2DM increased across both variables. Compared with participants with normal early-adulthood weight and BMI increase/decrease ≤1, the OR (95% CI) for T2DM of participants with early-adulthood overweight/obesity and BMI increase ≥4 kg/m(2) was 3.49 (3.05-4.00). For participants with early-adulthood underweight and BMI increase/decrease ≤ 1, the OR (95% CI) was 0.85 (0.75-0.97). Subgroup analysis according to sex and age showed similar trends. CONCLUSIONS: Early-adulthood BMI may influence T2DM prevalence after midlife independent of current BMI. T2DM prevalence after midlife was positively associated with early-adulthood weight gain and inversely related to early-adulthood weight loss, while early-adulthood weight loss could not completely negate the adverse effect of early-adulthood overweight/obesity on diabetes.

19 Article Relationship between Lipids Levels of Serum and Seminal Plasma and Semen Parameters in 631 Chinese Subfertile Men. 2016

Lu, Jin-Chun / Jing, Jun / Yao, Qi / Fan, Kai / Wang, Guo-Hong / Feng, Rui-Xiang / Liang, Yuan-Jiao / Chen, Li / Ge, Yi-Feng / Yao, Bing. ·The Reproductive Medical Center, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, China. · Department of Laboratory Science, Nanjing Hospital, Jiangsu Corps, The Armed Police Force, PLA, Nanjing, Jiangsu, China. ·PLoS One · Pubmed #26726884.

ABSTRACT: OBJECTIVE: This prospective study was designed to investigate the relationship between lipids levels in both serum and seminal plasma and semen parameters. METHODS: 631 subfertile men were enrolled. Their obesity-associated markers were measured, and semen parameters were analyzed. Also, seminal plasma and serum TC, TG, HDL and LDL and serum FFA, FSH, LH, total testosterone (TT), estradiol (E2) and SHBG levels were detected. RESULTS: Seminal plasma and serum TG, TC and LDL levels were positively related to age. Serum TC, TG and LDL were positively related to obesity-associated markers (P < 0.001), while only seminal plasma TG was positively related to them (P < 0.05). For lipids levels in serum and seminal plasma, only TG level had slightly positive correlation between them (r = 0.081, P = 0.042). There was no significant correlation between serum lipids levels and semen parameters. However, seminal plasma TG, TC, LDL and HDL levels were negatively related to one or several semen parameters, including semen volume (SV), sperm concentration (SC), total sperm count (TSC), sperm motility, progressive motility (PR) and total normal-progressively motile sperm counts (TNPMS). Moreover, seminal plasma TG, TC, LDL and HDL levels in patients with oligospermatism, asthenospermia and teratozoospermia were higher than those with normal sperm concentration, motility or morphology. After adjusting age and serum LH, FSH, TT, E2 and SHBG levels, linear regression analysis showed that SV was still significantly correlated with seminal plasma LDL (P = 0.012), both of SC and TSC with seminal plasma HDL (P = 0.028 and 0.002), and both of PR and sperm motility with seminal plasma TC (P = 0.012 and 0.051). CONCLUSION: The abnormal metabolism of lipids in male reproductive system may contribute to male factor infertility.

20 Article Association of the Number of Years Since Menopause with Metabolic Syndrome and Insulin Resistance in Chinese Urban Women. 2015

Yan, Fei / Liu, Jidong / Zhao, Xiangmin / Hu, Xiuping / Wang, Shaoyuan / Ma, Zeqiang / Liang, Kai / Song, Jun / Wang, Chuan / Hou, Xinguo / Chen, Shihong / Chen, Li. ·1 Department of Endocrinology of Qilu Hospital and Institute of Endocrinology and Metabolism, Shandong University , Jinan, China . · 7 Department of Poisoning and Occupational Diseases, Qilu Hospital of Shandong University , Jinan, China . · 2 Shantui Community Health Center , Jining, China . · 3 Department of Endocrinology, Second People's Hospital of Jining , Jining, China . · 4 Lukang Hospital of Jining , Jining, China . · 5 China National Heavy Duty Truck Group Corporation Hospital , Jinan, China . · 6 Department of Endocrinology, The Second Hospital of Shandong University , Jinan, Shandong, China . ·J Womens Health (Larchmt) · Pubmed #26418517.

ABSTRACT: BACKGROUND: This study aimed to assess the prevalence of metabolic syndrome (MetS) and the association of years since menopause with MetS and Insulin Resistance (IR) in Chinese women. METHOD: A total of 4436 Chinese subjects aged 40-80 years participated in the study; 790 were premenopausal women, and 3646 were postmenopausal women. IR was arbitrarily defined as a homeostasis model assessment-IR index (HOMA-IR) value above the 75th percentile of normal glucose tolerance (NGT). MetS was defined according to the International Diabetes Federation consensus definition. To test whether there was an association between the number of years since menopause and MetS, multivariate logistic analysis was conducted. Premenopausal women were used as a comparison group in regression analyses. RESULTS: After adjustment for age, body mass index (BMI), and γ-glutamyltransferase (GGT), more years since menopause was highly associated with an increased risk of MetS (p for trend <0.05) ; the number of years since menopause was not correlated with fasting insulin and HOMA-IR. Postmenopausal women with 10 to 14 years since menopause had the highest risk (odds ratio [OR], 2.10; 95% confidence interval [CI], 1.52-2.89, p < .05) of MetS, high triglycerides (TG; OR, 1.80; 95% CI, 1.34-2.42, p < .05) and high glucose (OR, 1.52; 95% CI, 1.14-2.05, p < .05) and low high-density lipoprotein cholesterol (HDL-C; OR, 1.38; 95% CI, 1.18-2.32, p < .05). Postmenopausal women with more than 15 years since menopause had the highest risk of abdominal obesity (OR, 1.69; 95% CI, 1.05-2.71, p < .05). CONCLUSION: In China, more years since menopause was highly associated with an increased risk of MetS. Menopausal history may help identify women with increased risk of developing MetS.

21 Article C-Peptide Is Independently Associated with an Increased Risk of Coronary Artery Disease in T2DM Subjects: A Cross-Sectional Study. 2015

Wang, Lingshu / Lin, Peng / Ma, Aixia / Zheng, Huizhen / Wang, Kexin / Li, Wenjuan / Wang, Chuan / Zhao, Ruxing / Liang, Kai / Liu, Fuqiang / Hou, Xinguo / Song, Jun / Lu, Yiran / Zhu, Ping / Sun, Yu / Chen, Li. ·Department of Endocrinology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, China. · Department of General Surgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012, China. · Department of Ophthalmology, College of medicine, University of Florida, Gainesville, FL 32610-0284, United States of America. · Department of Endocrinology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, China; Institute of Endocrinology and Metabolism, Shandong University, Jinan, Shandong 250012, China. ·PLoS One · Pubmed #26098780.

ABSTRACT: OBJECTIVE: C-peptide has been reported to be a marker of subclinical atherosclerosis in type 2 diabetes mellitus (T2DM) patients, whereas its role in coronary artery disease (CAD) has not been clarified, especially in diabetics with differing body mass indices (BMIs). DESIGN AND METHODS: This cross-sectional study included 501 patients with T2DM. First, all subjects were divided into the following two groups: CAD and non-CAD. Then, binary logistic regression was used to determine the risk factors for CAD for all patients. To clarify the role of obesity, we re-divided all subjects into two additional groups (obese and non-obese) based on BMI. Finally, binary logistic regression was used to determine the risk factors for CAD for each weight group. RESULTS: The patients with CAD showed a higher BMI and fasting C-peptide level in addition to an increased prevalence of traditional risk factors for CAD, such as hypertension, insulin resistance, higher cholesterol, cysteine-C (Cys-C) and lower estimated glomerular filtration rate (eGFR). Logistic regression analysis showed that fasting C-peptide (OR=1.513, p=0.005), insulin treatment (OR=1.832, p=0.027) hypertension (OR=1.987, p=0.016) and hyperlipidemia (OR=4.159, p<0.001) significantly increased the risk of clinical CAD in the T2DM patients independent of age, gender, diabetes duration, smoking and alcohol statuses, fasting insulin and glucose, hypoglycemic episodes, UA and eGFR. Additionally, in both of the obese (OR=1.488, p=0.049) and non-obese (OR=1.686, p=0.037) DM groups, C-peptide was associated with an increased risk of CAD after multiple adjustments. CONCLUSIONS: C-peptide is associated with an increased CAD risk in T2DM patients, no matter whether they are obese or not.

22 Article Fenofibrate treatment attenuated chronic endoplasmic reticulum stress in the liver of nonalcoholic fatty liver disease mice. 2015

Zhang, Nan / Lu, Yunxia / Shen, Xinru / Bao, Yingying / Cheng, Jingjing / Chen, Li / Li, Bao / Zhang, Qiu. ·Department of Endocrinology, First Affiliated Hospital of Anhui Medical University, Hefei, China. ·Pharmacology · Pubmed #25896720.

ABSTRACT: Fenofibrate is widely used in clinical practice, but its influence on chronic endoplasmic reticulum (ER) stress induced by feeding a high-calorie and high-cholesterol diet (HCD) has still not been studied. We thus investigated its effects on the liver of the nonalcoholic fatty liver disease (NAFLD) mouse model. Male C57BL/6 mice fed an HCD for 3 months were treated with fenofibrate (HCD + FF, 40 mg/kg, once daily) via gavage for 4 weeks. Insulin sensitivity, serum lipid and inflammatory cytokines were measured. Liver tissues were procured for histological examination as well as analysis of hepatic triglyceride levels, distribution of inflammatory cytokines and genes involved in ER stress. Our results showed that chronic feeding of an HCD successfully induced an NAFLD model accompanied by inflammatory activation, apoptosis and severe ER stress in the liver. Fenofibrate administration significantly improved symptoms of NAFLD and decreased apoptosis, expression of inflammatory cytokines and genes involved in ER stress, such as inositol-requiring enzyme 1α (IRE1α), X-box binding protein 1 (XBP1) and JNK phosphorylation. Thus, our study suggests that fenofibrate protected against inflammatory injury and apoptosis, maybe alleviating ER stress through the IRE1α-XBP1-JNK pathway in the liver of NAFLD mice.

23 Article Obesity and coronary artery calcification: Can it explain the obesity-paradox? 2015

Aljizeeri, Ahmed / Coutinho, Thais / Pen, Ally / Chen, Li / Yam, Yeung / Dent, Robert / McPherson, Ruth / Chow, Benjamin J W. ·Department of Medicine, University of Ottawa, Ottawa, Canada. ·Int J Cardiovasc Imaging · Pubmed #25788438.

ABSTRACT: The inverse relationship between obesity and adverse cardiovascular outcomes has been coined the 'obesity-paradox'. We sought to determine the relationship between measures of obesity [body mass index (BMI), body surface area (BSA) and body fat percentage (BF%)] and coronary artery calcification (CAC). We retrospectively analyzed patients who underwent CAC using the Agatston score. Baseline demographics were collected and BMI, BSA and BF% were calculated. A two-stage regression modeling approach was used to evaluate the association between BMI, BSA, BF% and Agatston score. Of the 6661 patients [mean age = 57.1 ± 10.8 years, men = 54.3%, median Agatston score = 14 (0, 163)], 0.1% were underweight, 21.3% had normal BMI, 39.1% were overweight and 39.4% were obese. The mean BMI, BSA and BF% were 29.6 ± 6.1 kg/m(2), 1.97 ± 0.25 m(2) and 37 ± 10 %, respectively. There was an independent association between the presence of CAC and BMI (5 kg/m(2) increments) (OR 1.05, CI 1.00-1.11, P = 0.038) and BF% (OR 2.38, CI 1.05-5.41, P = 0.038). Neither BMI categories nor large BSA independently predicted the presence of CAC. BF% predicted the extent of CAC in men but not in women, and higher BF% was associated with higher category of CAC severity in men only. BMI and BF% were independent predictors of the presence of CAC. BF% was associated with the extent of CAC and higher BF% was associated with higher category of CAC severity in men only. These results suggest that further study is needed to better understand the obesity-paradox.

24 Article Endoplasmic reticulum stress involved in high-fat diet and palmitic acid-induced vascular damages and fenofibrate intervention. 2015

Lu, Yunxia / Cheng, Jingjing / Chen, Li / Li, Chaofei / Chen, Guanjun / Gui, Li / Shen, Bing / Zhang, Qiu. ·Department of Biochemistry and Molecular Biology, Anhui Medical University, Hefei, Anhui 230032, PR China; The Comprehensive Laboratory, Anhui Medical University, Hefei, Anhui 230032, PR China. Electronic address: wwwdluyx@sina.com. · Department of Biochemistry and Molecular Biology, Anhui Medical University, Hefei, Anhui 230032, PR China. · Department of Biochemistry and Molecular Biology, Anhui Medical University, Hefei, Anhui 230032, PR China; Department of Medical Laboratory, Anhui Provincial Hospital, Hefei, Anhui 230001, PR China. · The Comprehensive Laboratory, Anhui Medical University, Hefei, Anhui 230032, PR China. · Department of Physiology, Anhui Medical University, Hefei, Anhui 230032, PR China. · Department of Endocrinology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, PR China. ·Biochem Biophys Res Commun · Pubmed #25592967.

ABSTRACT: Fenofibrate (FF) is widely used to lower blood lipids in clinical practice, but whether its protective effect on endothelium-dependent vasodilatation (EDV) in thoracic aorta is related with endoplasmic reticulum (ER) stress remains unknown. In this study, female Sprauge Dawley rats were divided into standard chow diets (SCD), high-fat diets (HFD) and HFD plus FF treatment group (HFD + FF) randomly. The rats of latter two groups were given HFD feeding for 5 months, then HFD + FF rats were treated with FF (30 mg/kg, once daily) via gavage for another 2 months. The pathological and tensional changes, protein expression of eNOS, and ER stress related genes in thoracic aorta were measured. Then impacts of palmitic acid (PA) and FF on EDV of thoracic aorta from normal female SD rats were observed. Ultimately the expression of ER stress related genes were assessed in primary mouse aortic endothelial cells (MAEC) treated by fenofibric acid (FA) and PA. We found that FF treatment improved serum lipid levels and pathological changes in thoracic aorta, accompanied with decreased ER stress and increased phosphorylation of eNOS. FF pretreatment also improved EDV impaired by different concentrations of PA treatment. The dose- and time-dependent inhibition of cell proliferation by PA were inverted by FA pretreatment. Phosphorylation of eNOS and expression of ER stress related genes were all inverted by FA pretreatment in PA-treated MAEC. Our findings show that fenofibrate recovers damaged EDV by chronic HFD feeding and acute stimulation of PA, this effect is related with decreased ER stress and increased phosphorylation of eNOS.

25 Article Age and sex-specific relationships between phthalate exposures and obesity in Chinese children at puberty. 2014

Zhang, Yunhui / Meng, Xiangzhou / Chen, Li / Li, Dan / Zhao, Lifang / Zhao, Yan / Li, Luxi / Shi, Huijing. ·Department of Environment Health, School of Public Health/Key Laboratory of Public Health Safety of Chinese Ministry of Education, Fudan University, Shanghai, China. · State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, China. · Department of Maternal, Child and Adolescent Health, School of Public Health/Key Laboratory of Public Health Safety of Chinese Ministry of Education, Fudan University, Shanghai, China. ·PLoS One · Pubmed #25121758.

ABSTRACT: OBJECTIVE: To examine the age and sex-specific associations of urine levels of six mono-phthalates with body size and fat distribution in Chinese children at puberty. MATERIALS AND METHODS: Four hundred and ninety-three school-aged children (247 boys, 246 girls) were recruited. Obesity related anthropometric indices were measured and body fat proportion (BF%) was calculated. Spot urine samples were collected and phthalate monoesters were detected by an API 2000 electrospray triple quadrupole mass spectrometer (ESI-MS/MS). Associations between phthalate exposure and overweight/obesity measures and their trends were examined by multiple linear regression and Logistic regression analyses, respectively. RESULTS: Di-2-ethylhexyl phthalate (DEHP) metabolites and monobutyl phthalate (MBP) were found to be the most detectable chemicals. In 8-10 years (yrs) group, concentrations of MEHP and MBP were significantly higher in girls than those in boys. However, concentrations of all phthalate monoesters, except for MEP and MEHP, in 11-13 yrs boys were significantly higher than those in girls. After adjusting for confounders including puberty onset, urinary concentrations of MBP and sum of low molecular-weight phthalate metabolites (∑LMP) were positively associated with boys' obesity in a concentration-effect manner, while concentrations of MEHP, MEHHP and sum of DEHP metabolites (∑MEHP) were negatively associated with girls' obesity. Associations between phthalate exposure levels and BMI z-score changes were age- and sex-specific in school-age children. CONCLUSION: There are age and sex-specific concentration-effect associations between phthalate exposure and fat distribution in Chinese children. Urinary phthalate levels in 11-13 yrs boys were about 30 percent higher than those in girls, and ∑MEHP levels in younger boys (<10 yrs) were significantly higher than those in elder boys (>10 yrs). Associations were positive for MBP and ∑LMP with both BMI z-score and fat distribution in boys >10 years of age, and negative for ∑MEHP with fat distribution in girls <10 years of age.

Next